Centro de Investigacion en Alimentacion y Desarrollo, AC, Carretera a la Victoria km. 0.6, Hermosillo 83000, Mexico.
Universidad de Sonora, Blvd. Luis Encinas y Rosales S/N, Col. Centro, Hermosillo, Sonora 83000, Mexico.
Molecules. 2018 Oct 30;23(11):2813. doi: 10.3390/molecules23112813.
The objective of this study was to evaluate the effect of combining catechin, protocatechuic, and vanillic acids against planktonic growing, adhesion, and biofilm eradication of uropathogenic (UPEC), as well as antioxidant agents. The minimum inhibitory concentrations (MIC) of protocatechuic, vanillic acids and catechin against the growth of planktonic bacteria were 12.98, 11.80, and 13.78 mM, respectively. Mixing 1.62 mM protocatechuic acid + 0.74 mM vanillic acid + 0.05 mM catechin resulted in a synergistic effect acting as an MIC. Similarly, the minimum concentrations of phenolic compounds to prevent UPEC adhesion and biofilm formation (MBIC) were 11.03 and 7.13 mM of protocatechuic and vanillic acids, respectively, whereas no MBIC of catechin was found. However, combinations of 1.62 mM protocatechuic acid + 0.74 mM vanillic acid + 0.05 mM catechin showed a synergistic effect acting as MBIC. On the other hand, the minimum concentrations to eradicate biofilms (MBEC) were 25.95 and 23.78 mM, respectively. The combination of 3.20 mM protocatechuic acid, 2.97 mM vanillic acid, and 1.72 mM catechin eradicated pre-formed biofilms. The antioxidant capacity of the combination of phenolics was higher than the expected theoretical values, indicating synergism by the DPPH, ABTS, and FRAP assays. Effective concentrations of catechin, protocatechuic, and vanillic acids were reduced from 8 to 1378 times when combined. In contrast, the antibiotic nitrofurantoin was not effective in eradicating biofilms from silicone surfaces. In conclusion, the mixture of phenolic compounds was more effective in preventing cell adhesion and eradicating pre-formed biofilms of uropathogenic than single compounds and nitrofurantoin, and showed antioxidant synergy.
本研究旨在评估儿茶素、原儿茶酸和香草酸联合使用对浮游生长、黏附和生物膜清除的影响，以及抗氧化剂的影响。原儿茶酸、香草酸和儿茶素对浮游菌生长的最小抑菌浓度（MIC）分别为 12.98、11.80 和 13.78mM。混合 1.62mM 原儿茶酸+0.74mM 香草酸+0.05mM 儿茶素可协同作用，MIC 值为 1.62mM。同样，酚类化合物预防 UPEC 黏附和生物膜形成的最小浓度（MBIC）分别为 11.03 和 7.13mM 的原儿茶酸和香草酸，而儿茶素则没有 MBIC。然而，1.62mM 原儿茶酸+0.74mM 香草酸+0.05mM 儿茶素的组合表现出协同作用，MBIC 值为 1.62mM。另一方面，清除生物膜的最低浓度（MBEC）分别为 25.95 和 23.78mM。3.20mM 原儿茶酸、2.97mM 香草酸和 1.72mM 儿茶素的组合可根除已形成的生物膜。酚类混合物的抗氧化能力高于预期的理论值，表明 DPPH、ABTS 和 FRAP 测定法具有协同作用。儿茶素、原儿茶酸和香草酸的有效浓度在联合使用时降低了 8 至 1378 倍。相比之下，抗生素呋喃妥因在清除硅酮表面的生物膜方面无效。总之，与单一化合物和呋喃妥因相比，酚类混合物更有效地预防尿路感染生物膜的细胞黏附和根除，表现出抗氧化协同作用。
