Vale J, Ribeiro M, Abreu A C, Soares-Silva I, Simões M
LEPABE, Department of Chemical Engineering, Faculty of Engineering, University of Porto, Porto, Portugal.
i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Porto, Portugal.
Lett Appl Microbiol. 2019 Apr;68(4):313-320. doi: 10.1111/lam.13137. Epub 2019 Mar 13.
The aim of this study was the development of a novel and effective antibacterial formulation combining selected phytochemical compounds (quercetin, cuminaldehyde, indole-3-carbinol and vanillic acid) with ethylenediaminetetraacetic acid (EDTA), an aminopolycarboxylic acid. The antibacterial activity of the combinations was evaluated against Escherichia coli and Staphylococcus epidermidis in planktonic and sessile states as single and dual species. The compounds when applied individually demonstrated modest antibacterial activity. Nevertheless, synergy was observed when EDTA was combined with the selected phytochemicals, particularly with cuminaldehyde and indole-3-carbinol. These combinations were evaluated against single- and dual-species biofilms. An inactivation of 100% was obtained for almost all the biofilms, with E. coli biofilms showing the highest resistance. This study allowed the discovery of novel formulations of phytochemical compounds with antibacterial activity against E. coli and S. epidermidis single- and dual-species biofilms at concentrations close to the minimum bactericidal concentration. SIGNIFICANCE AND IMPACT OF THE STUDY: The synergistic combinations of EDTA and cuminaldehyde or indole-3-carbinol were effective against single- and dual-species E. coli and S. epidermidis planktonic cells and biofilms. The overall results highlight the role of phytochemical products as a green and sustainable source of antimicrobial potentiators to control bacteria in both planktonic and sessile states.
本研究的目的是开发一种新型有效的抗菌制剂，该制剂将选定的植物化学化合物（槲皮素、枯茗醛、吲哚 - 3 - 甲醇和香草酸）与氨基多羧酸乙二胺四乙酸（EDTA）相结合。评估了这些组合在浮游态和固着态下对大肠杆菌和表皮葡萄球菌单物种及双物种的抗菌活性。这些化合物单独应用时表现出适度的抗菌活性。然而，当EDTA与选定的植物化学物质，特别是与枯茗醛和吲哚 - 3 - 甲醇组合时，观察到了协同作用。对这些组合进行了针对单物种和双物种生物膜的评估。几乎所有生物膜都实现了100%的失活，其中大肠杆菌生物膜表现出最高的抗性。本研究发现了新型的植物化学化合物制剂，其在接近最低杀菌浓度的浓度下对大肠杆菌和表皮葡萄球菌单物种及双物种生物膜具有抗菌活性。研究的意义和影响：EDTA与枯茗醛或吲哚 - 3 - 甲醇的协同组合对单物种和双物种的大肠杆菌和表皮葡萄球菌浮游细胞及生物膜有效。总体结果突出了植物化学产品作为绿色可持续抗菌增效剂来源在控制浮游态和固着态细菌方面的作用。
