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The structure of the promoter and amino terminal region of the pH 2.5 acid phosphatase structural gene (appA) of E. coli: a negative control of transcription mediated by cyclic AMP.

作者信息

Touati E, Danchin A

出版信息

Biochimie. 1987 Mar;69(3):215-21. doi: 10.1016/0300-9084(87)90045-9.

DOI:10.1016/0300-9084(87)90045-9
PMID:3038201
Abstract

The regulatory and promoter regions of the gene for acid phosphatase of optimum pH 2.5 (appA) of Escherichia coli has been characterized by constructing appA-lacZ protein fusions. Monitoring of beta-galactosidase activity showed that the cloned fragment harbored a region that was responsible for a negative control of transcription mediated by the cAMP-cAMP receptor (CAP) complex. The nucleotide sequence of the segment was determined. A region containing a putative CAP binding site, overlapping the -10 region of the promoter was found. Deletion analysis around the promoter region, using appA-lacZ protein fusions substantiated the hypothesis for a role of the 'consensus' sequence in the negative control mediated by cyclic AMP. In addition, the coding sequence revealed the likely existence of a signal peptide similar to the one found for other exported proteins, upstream from the phosphatase protein sequence.

摘要

相似文献

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