Department of Psychology and Neuroscience, Life Sciences Centre, Dalhousie University, Halifax, Canada.
Janelia Research Campus, Howard Hughes Medical Institute, Virginia, United States.
Elife. 2018 Nov 1;7:e37550. doi: 10.7554/eLife.37550.
Using FIB-SEM we report the entire synaptic connectome of glomerulus VA1v of the right antennal lobe in . Within the glomerulus we densely reconstructed all neurons, including hitherto elusive local interneurons. The -positive, sexually dimorphic VA1v included >11,140 presynaptic sites with ~38,050 postsynaptic dendrites. These connected input olfactory receptor neurons (ORNs, 51 ipsilateral, 56 contralateral), output projection neurons (18 PNs), and local interneurons (56 of >150 previously reported LNs). ORNs are predominantly presynaptic and PNs predominantly postsynaptic; newly reported LN circuits are largely an equal mixture and confer extensive synaptic reciprocity, except the newly reported LN2V with input from ORNs and outputs mostly to monoglomerular PNs, however. PNs were more numerous than previously reported from genetic screens, suggesting that the latter failed to reach saturation. We report a matrix of 192 bodies each having 50 connections; these form 88% of the glomerulus' pre/postsynaptic sites.
利用 FIB-SEM，我们报告了右触角叶 VA1v 肾小球的整个突触连接组。在肾小球内，我们密集重建了所有神经元，包括迄今难以捉摸的局部中间神经元。FA 阳性、性二态性的 VA1v 包括 >11140 个突触前位点和~38050 个突触后树突。这些连接了输入嗅觉受体神经元（ORNs，51 个同侧，56 个对侧）、输出投射神经元（18 个 PN）和局部中间神经元（56 个先前报道的 LN 中的 >150 个）。ORNs 主要是突触前的，PNs 主要是突触后的；新报道的 LN 回路主要是均等混合的，并赋予广泛的突触相互作用，除了新报道的 LN2V，其输入来自 ORNs，输出主要到单肾小球 PN。然而，PN 的数量比先前的遗传筛选报告的要多，这表明后者未能达到饱和。我们报告了一个包含 50 个连接的 192 个体矩阵；这些构成了肾小球的 88%的突触前/后位点。
