Department of Environmental Chemistry, Faculty of Chemistry, Jagiellonian University, Gronostajowa 2, 30-387, Kraków, Poland.
Department of Physical Chemistry and Electrochemistry, Faculty of Chemistry, Jagiellonian University, Gronostajowa 2, 30-387, Kraków, Poland.
Colloids Surf B Biointerfaces. 2019 Jan 1;173:672-680. doi: 10.1016/j.colsurfb.2018.10.047. Epub 2018 Oct 19.
Many plant-derived compounds possess antimicrobial, antioxidant and even anticancer activities. Therefore, they are considered as substances that can be used instead of synthetic compounds in various applications. In this work, the essential oil from hop cones was extracted and analyzed, and then its effects on model bacteria membranes were studied to verify whether the hop essential oils could be used as ecological pesticides. The experiments involved surface pressure-area measurements, penetration studies and Brewster angle microscopy (BAM) imaging of lipid monolayers as well as hydrodynamic diameter, zeta potential, steady-state fluorescence anisotropy and Cryo-Transmission Electron Microscopy (cryo-TEM) measurements of liposomes. Finally the bactericidal tests on plant pathogen bacteria Pseudomonas syringae pv. lachrymans PCM 1410 were performed. The obtained results showed that the components of the essential oils from hop cones incorporate into lipid monolayers and bilayers and alter their fluidity. However, the observed effect is determined by the system composition, its condensation and the oil concentration. Interestingly, at a given dose, the effect of the essential oil on membranes was found to stabilize. Moreover, BAM images proved that hop oil prevents the formation of a large fraction of a condensed phase at the interface. Both the studies on model membranes as well as the in vitro tests allow one to conclude that the hop essential oil could likely be considered as the candidate to be used in agriculture as a natural pesticide.
许多植物衍生化合物具有抗菌、抗氧化甚至抗癌活性。因此，它们被认为是可以在各种应用中替代合成化合物的物质。在这项工作中，提取并分析了啤酒花穗的精油，并研究了其对模型细菌膜的作用，以验证啤酒花精油是否可作为生态农药使用。实验涉及表面压力-面积测量、穿透研究以及脂质单层的布鲁斯特角显微镜 (BAM) 成像，以及脂质体的水动力直径、zeta 电位、稳态荧光各向异性和冷冻传输电子显微镜 (cryo-TEM) 测量。最后对植物病原菌丁香假单胞菌 pv. lachrymans PCM 1410 进行了杀菌测试。结果表明，啤酒花穗精油的成分掺入脂质单层和双层中并改变其流动性。然而，观察到的效果取决于系统组成、其浓缩和油浓度。有趣的是，在给定剂量下，发现精油对膜的作用具有稳定作用。此外，BAM 图像证明啤酒花油可防止在界面形成大量浓缩相。对模型膜的研究以及体外测试均表明，啤酒花精油很可能被认为是农业中用作天然农药的候选物。
