臭荠精油：化学成分、多变量分析及抗菌活性

Dittrichia graveolens (L.) Greuter Essential Oil: Chemical Composition, Multivariate Analysis, and Antimicrobial Activity.

作者信息

Mitic Violeta, Stankov Jovanovic Vesna, Ilic Marija, Jovanovic Olga, Djordjevic Aleksandra, Stojanovic Gordana

机构信息

Department of Chemistry, Faculty of Science and Mathematics, University of Niš, Višegradska 33, SR-18000 Niš, (phone: +381-63-1094273; fax: +381-18-533014).

出版信息

Chem Biodivers. 2016 Jan;13(1):85-90. doi: 10.1002/cbdv.201500028.

DOI:10.1002/cbdv.201500028

PMID:26765355

Abstract

The chemical composition and in vitro antimicrobial activities of Dittrichia graveolens (L.) Greuter essential oil was studied. Moreover, using agglomerative hierarchical cluster (AHC) and principal component analyses (PCA), the interrelationships of the D. graveolens essential-oil profiles characterized so far (including the sample from this study) were investigated. To evaluate the chemical composition of the essential oil, GC-FID and GC/MS analyses were performed. Altogether, 54 compounds were identified, accounting for 92.9% of the total oil composition. The D. graveolens oil belongs to the monoterpenoid chemotype, with monoterpenoids comprising 87.4% of the totally identified compounds. The major components were borneol (43.6%) and bornyl acetate (38.3%). Multivariate analysis showed that the compounds borneol and bornyl acetate exerted the greatest influence on the spatial differences in the composition of the reported oils. The antimicrobial activity against five bacterial and one fungal strain was determined using a disk-diffusion assay. The studied essential oil was active only against Gram-positive bacteria.

摘要

研究了香叶泽兰（Dittrichia graveolens (L.) Greuter）精油的化学成分和体外抗菌活性。此外，使用凝聚层次聚类（AHC）和主成分分析（PCA），研究了迄今为止所表征的香叶泽兰精油谱（包括本研究中的样品）之间的相互关系。为了评估精油的化学成分，进行了气相色谱-火焰离子化检测（GC-FID）和气相色谱/质谱（GC/MS）分析。总共鉴定出54种化合物，占总油成分的92.9%。香叶泽兰油属于单萜类化学型，单萜类化合物占已鉴定化合物总数的87.4%。主要成分是冰片（43.6%）和乙酸龙脑酯（38.3%）。多变量分析表明，冰片和乙酸龙脑酯对所报道油类成分的空间差异影响最大。使用纸片扩散法测定了对五种细菌菌株和一种真菌菌株的抗菌活性。所研究的精油仅对革兰氏阳性菌有活性。