State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai 200050, China; University of Chinese Academy of Sciences, Beijing 100049, China.
State Key Laboratory of Functional Materials for Informatics, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai 200050, China; University of Chinese Academy of Sciences, Beijing 100049, China.
Colloids Surf B Biointerfaces. 2019 Jan 1;173:681-688. doi: 10.1016/j.colsurfb.2018.10.050. Epub 2018 Oct 21.
Graphene halides are promising two-dimensional systems which have interesting physical and chemical properties. In particular, high quality fluorinated graphene offers a great potential in modulating variable properties by regulating its surface microstructure. Moreover, the fluorine introduction and carbon-fluorine bonding characters will enable some interesting biological response. Here, the biological responses to bacteria and cells of fluorinated graphene were studied. Present work revealed that partially fluorinated graphene behaved satisfactory antibacterial ability. Fluorinated graphene showed well facilitating function to cell adhesion in early period, however, after a longer incubation period, the enhanced intracellular ROS level in rBMSCs on the fully fluorinated graphene gives rise to the decrease of cell viability. It was found that there is no statistical difference in the activity of alkaline phosphatase (ALP) and matrix mineralization of rBMSCs on pristine graphene, partially fluorinated graphene and fluorographene. In addition, the introduction of fluorine into pristine graphene plane reduced the adhesion and aggregation of blood platelets due to the attenuation of π-π interaction between material surface and blood protein. The findings in this work revealed that partial fluorinated graphene exhibited better antibacterial ability and cytocompatibility, outperforming pristine graphene and fluorographene.
石墨烯卤化物是一种很有前途的二维体系，具有有趣的物理和化学性质。特别是高质量的氟化石墨烯在通过调节其表面微观结构来调节可变性质方面具有很大的潜力。此外，氟的引入和碳-氟键合特性将使一些有趣的生物学反应成为可能。在这里，研究了氟化石墨烯对细菌和细胞的生物学反应。目前的工作表明，部分氟化石墨烯具有令人满意的抗菌能力。氟化石墨烯在早期对细胞黏附具有很好的促进作用，但在较长的孵育时间后，rBMSCs 中完全氟化石墨烯上增强的细胞内 ROS 水平导致细胞活力下降。结果发现，在原石墨烯、部分氟化石墨烯和氟化石墨烯上，rBMSCs 的碱性磷酸酶（ALP）活性和基质矿化没有统计学差异。此外，由于材料表面与血液蛋白之间的π-π相互作用减弱，氟原子引入到原石墨烯平面上减少了血小板的黏附和聚集。这项工作的结果表明，部分氟化石墨烯表现出更好的抗菌能力和细胞相容性，优于原石墨烯和氟化石墨烯。
