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一类新型I型限制酶对DNA的识别：两种不同DNA特异性之间的独特关系。

DNA recognition by a new family of type I restriction enzymes: a unique relationship between two different DNA specificities.

作者信息

Price C, Shepherd J C, Bickle T A

出版信息

EMBO J. 1987 May;6(5):1493-7. doi: 10.1002/j.1460-2075.1987.tb02391.x.

DOI:10.1002/j.1460-2075.1987.tb02391.x
PMID:3038525
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC553956/
Abstract

The DNA sequences recognized by the allelic type I restriction enzymes EcoR124 and EcoR124/3 were determined. EcoR124 recognizes 5'-GAA(N6)RTCG-3' and EcoR124/3 recognizes 5'-GAA(N7)RTCG-3'. These are typical of sequences recognized by type I recognition enzymes in that they consist of two specific domains separated by a non-specific spacer sequence. For these two enzymes, the specific sequences are identical but the length of the non-specific spacer is different. The specific domains of EcoR124/3 are thus 3.4 A further apart than those of EcoR124 and rotated with respect to each other through a further 36 degrees.

摘要

确定了等位基因I型限制酶EcoR124和EcoR124/3识别的DNA序列。EcoR124识别5'-GAA(N6)RTCG-3'，EcoR124/3识别5'-GAA(N7)RTCG-3'。这些是I型识别酶识别的典型序列，因为它们由两个特定结构域组成，中间由一个非特异性间隔序列隔开。对于这两种酶，特定序列相同，但非特异性间隔的长度不同。因此，EcoR124/3的特定结构域比EcoR124的特定结构域相距更远3.4 Å，并且彼此相对旋转了36度。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad86/553956/572c966dac46/emboj00245-0334-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad86/553956/8fc752d07d8c/emboj00245-0333-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad86/553956/a0889a4301e6/emboj00245-0333-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad86/553956/3ef9457cdb93/emboj00245-0334-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad86/553956/572c966dac46/emboj00245-0334-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad86/553956/8fc752d07d8c/emboj00245-0333-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad86/553956/a0889a4301e6/emboj00245-0333-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad86/553956/3ef9457cdb93/emboj00245-0334-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad86/553956/572c966dac46/emboj00245-0334-b.jpg

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