Price C, Pripfl T, Bickle T A
Eur J Biochem. 1987 Aug 17;167(1):111-5. doi: 10.1111/j.1432-1033.1987.tb13310.x.
We have purified the EcoR124 and EcoR124/3 restriction enzymes and shown that they are type I enzymes by several criteria: subunit composition, DNA and S-adenosylmethionine-dependent ATPase activity, and site-specific DNA methylase activity. By immunochemical criteria these enzymes are related to each other but are unrelated to the two previously investigated families of type I restriction enzymes. They form therefore a new family which we call type IC. The arrangement of the structural genes coding for these enzymes and their transcriptional organisation have been determined. These are different from the common arrangement found for the other two families of type I enzymes.
我们已经纯化了EcoR124和EcoR124/3限制性内切酶，并通过几个标准表明它们是I型酶：亚基组成、依赖DNA和S-腺苷甲硫氨酸的ATP酶活性以及位点特异性DNA甲基化酶活性。根据免疫化学标准，这些酶彼此相关，但与之前研究的两个I型限制性内切酶家族无关。因此，它们形成了一个新的家族，我们称之为IC型。已经确定了编码这些酶的结构基因的排列及其转录组织。这些与另外两个I型酶家族的常见排列不同。
