Passananti C, Davies B, Ford M, Fried M
EMBO J. 1987 Jun;6(6):1697-703. doi: 10.1002/j.1460-2075.1987.tb02420.x.
Inverted duplications have been observed to be a common feature of gene amplification in mammalian cells and appear to be generated as a primary event in the amplification process (Ford et al., 1985; Ford and Fried, 1986). The structural features of the amplified inverted duplication, containing the polyoma virus oncogene middle T-antigen, were analysed in transformed 3B rat cells. No unusual sequences such as transposition elements were detected at the site of the inversion. The inversion was generated by a simple illegitimate recombination event in which only a single nucleotide directly at the point of the inversion cannot be accounted for from the sequence of the two parental strands. Possible structural (hairpin formation) and sequence (rich AT) features may have been involved in the illegitimate recombination event at the inversion join. In the cellular DNA near one of its joins with polyoma virus DNA an unusual sequence of 198 bp composed of 99 consecutive purine-pyrimidine pairs has been detected. A model for the generation of amplified DNA containing inverted duplications is proposed.
倒位重复已被观察到是哺乳动物细胞基因扩增的一个常见特征，并且似乎是在扩增过程中作为一个主要事件产生的（福特等人，1985年；福特和弗里德，1986年）。在转化的3B大鼠细胞中分析了包含多瘤病毒癌基因中T抗原的扩增倒位重复的结构特征。在倒位位点未检测到转座元件等异常序列。倒位是由一个简单的非法重组事件产生的，在该事件中，仅倒位点处的单个核苷酸无法从两条亲代链的序列中得到解释。可能的结构（发夹形成）和序列（富含AT）特征可能参与了倒位连接处的非法重组事件。在其与多瘤病毒DNA的一个连接处附近的细胞DNA中，检测到了一个由99个连续嘌呤-嘧啶对组成的198 bp异常序列。提出了一个包含倒位重复的扩增DNA产生模型。
