Williams T J, Fried M
Mol Cell Biol. 1986 Jun;6(6):2179-84. doi: 10.1128/mcb.6.6.2179-2184.1986.
Illegitimate recombination events in mammalian cells often contain extraneous nucleotides or filler DNA at the recombinant joins. The polyomavirus-transformed cell line 7axB has previously been found to contain 37 base pairs (bp) of filler DNA at one virus-host join of the single insert of integrated viral DNA (A. Hayday, H. E. Ruley, and M. Fried, J. Virol. 44:67-77, 1982). By using a synthetic oligomer of these 37 bp as a probe, we demonstrated that this filler DNA is an inverted duplication of a single-copy rat sequence found 650 bp upstream from this virus-host join. The other virus-host join appears to be the result of a simple illegitimate recombination event between viral and host sequences. This is the first identification of filler DNA as a transposed copy of a chromosomal sequence. The relevance of the recombination events studied to cellular rearrangements and viral integration is discussed.
哺乳动物细胞中的非法重组事件在重组连接处常常包含外来核苷酸或填充DNA。先前已发现多瘤病毒转化的细胞系7axB在整合病毒DNA的单拷贝插入片段的一个病毒-宿主连接处含有37个碱基对（bp）的填充DNA（A. 海迪、H. E. 鲁利和M. 弗里德，《病毒学杂志》44:67 - 77, 1982年）。通过使用这37 bp的合成寡聚物作为探针，我们证明这种填充DNA是在此病毒-宿主连接处上游650 bp处发现的单拷贝大鼠序列的反向重复。另一个病毒-宿主连接处似乎是病毒和宿主序列之间简单非法重组事件的结果。这是首次将填充DNA鉴定为染色体序列的转座拷贝。讨论了所研究的重组事件与细胞重排和病毒整合的相关性。
