N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russian Federation.
N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russian Federation.
Int J Biol Macromol. 2019 Feb 1;122:555-561. doi: 10.1016/j.ijbiomac.2018.10.189. Epub 2018 Oct 29.
A branched O-polysaccharide was obtained by mild acid degradation of the lipopolysaccharide of Yersinia rohdei H274-36/78 and found to contain d-rhamnose, d-mannose, and 3,6-dideoxy-4-C-[(S)-1-hydroxyethyl]-d-xylo-hexose called yersiniose A (Yer). Partial acid hydrolysis of the O-polysaccharide eliminated Yer residues to give a modified linear polysaccharide. Studies by sugar analysis and H and C NMR spectroscopy, including computational NMR analysis, enabled structure elucidation of a hexasaccharide repeating unit of the O-polysaccharide having two Yer residues attached as monosaccharide side chains. The O-antigen gene cluster of Y. rohdei H274-36/78 located between JUMPStart and galF genes contained putative genes for synthesis of precursors of two O-antigen constituents, GDP-d-Man and GDP-d-Rha, whereas genes responsible for synthesis of CDP-Yer were within the chromosome outside the O-antigen gene cluster. Glycosyltransferase genes and ABC 2 transporter genes were present in the O-antigen gene cluster, and hence the structure established is consistent with the polysaccharide synthesis gene content of the genome.
一株分枝 O-多糖通过温和酸降解罗赫氏耶尔森菌 H274-36/78 的脂多糖获得，该多糖含有 D-鼠李糖、D-甘露糖和 3,6-二脱氧-4-C-[(S)-1-羟基乙基]-D-木己糖，称为耶尔森氏糖 A (Yer)。O-多糖的部分酸水解消除了 Yer 残基，得到了一种修饰的线性多糖。通过糖分析和 H 和 C NMR 光谱研究，包括计算 NMR 分析，阐明了 O-多糖的六糖重复单元的结构，该重复单元有两个 Yer 残基作为单糖侧链连接。罗赫氏耶尔森菌 H274-36/78 的 O-抗原基因簇位于 JUMPStart 和 galF 基因之间，包含合成两种 O-抗原成分 GDP-d-Man 和 GDP-d-Rha 前体的假定基因，而负责合成 CDP-Yer 的基因则位于染色体上 O-抗原基因簇之外。糖基转移酶基因和 ABC 2 转运体基因存在于 O-抗原基因簇中，因此建立的结构与基因组中多糖合成基因的含量一致。
