Sizova Olga V, Shashkov Alexander S, Kondakova Anna N, Knirel Yuriy A, Shaikhutdinova Rima Z, Ivanov Sergei A, Kislichkina Angelina A, Kadnikova Lidia A, Bogun Aleksandr G, Dentovskaya Svetlana V
N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninskii Prospekt 47, 119991 Moscow, Russia.
N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninskii Prospekt 47, 119991 Moscow, Russia.
Carbohydr Res. 2018 May 2;460:51-56. doi: 10.1016/j.carres.2018.02.014. Epub 2018 Feb 28.
Lipopolysaccharide was isolated from bacteria Yersinia intermedia H9-36/83 (O:17) and degraded with mild acid to give an O-specific polysaccharide, which was isolated by GPC on Sephadex G-50 and studied by sugar analysis and 1D and 2D NMR spectroscopy. The polysaccharide was found to contain 3-deoxy-3-[(R)-3-hydroxybutanoylamino]-d-fucose (d-Fuc3NR3Hb) and the following structure of the heptasaccharide repeating unit was established: The structure established is consistent with the gene content of the O-antigen gene cluster. The O-polysaccharide structure and gene cluster of Y. intermedia are related to those of Hafnia alvei 1211 and Escherichia coli O:103.
从中间耶尔森菌H9-36/83（O:17）中分离出脂多糖，并用稀酸降解得到O-特异性多糖，通过在葡聚糖凝胶G-50上进行凝胶渗透色谱法（GPC）分离该多糖，并通过糖分析以及一维和二维核磁共振光谱进行研究。发现该多糖含有3-脱氧-3-[(R)-3-羟基丁酰氨基]-D-岩藻糖（d-Fuc3NR3Hb），并确定了七糖重复单元的以下结构：所确定的结构与O-抗原基因簇的基因内容一致。中间耶尔森菌的O-多糖结构和基因簇与蜂房哈夫尼亚菌1211和大肠杆菌O:103的相关。
