Heikes Kira L, Goldstein Bob
Biology Department, University of North Carolina, Chapel Hill, North Carolina 27599.
Curriculum in Genetics and Molecular Biology, University of North Carolina, Chapel Hill, North Carolina 27599.
Cold Spring Harb Protoc. 2018 Nov 1;2018(11):2018/11/pdb.prot102335. doi: 10.1101/pdb.prot102335.
The tardigrade was chosen as a model system in part because embryos and animals are optically clear at all stages, facilitating the viewing and filming of internal processes. Multiplane video recordings under differential interference contrast (DIC) microscopy have allowed early embryonic cell lineages to be reconstructed through seven rounds of division and have revealed invariant patterns of asymmetric cell divisions, nuclear migrations, and cell migrations. Here, we present a protocol for filming embryonic development of by DIC microscopy.
选择缓步动物作为模型系统，部分原因是其胚胎和动物在所有阶段都是光学透明的，便于观察和拍摄内部过程。在微分干涉差（DIC）显微镜下进行的多平面视频记录，使得早期胚胎细胞谱系能够通过七轮分裂进行重建，并揭示了不对称细胞分裂、核迁移和细胞迁移的不变模式。在此，我们展示了一种通过DIC显微镜拍摄胚胎发育过程的方案。
