Smith Frank W, Gabriel Willow N
Biology Department, University of North Florida, Jacksonville, Florida 32224;
Department of Biology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599.
Cold Spring Harb Protoc. 2018 Nov 1;2018(11):2018/11/pdb.prot102343. doi: 10.1101/pdb.prot102343.
Immunostaining is a method used to visualize the localization of proteins in fixed tissue. Many antibodies are available that recognize specific proteins in a wide diversity of organisms, which makes this method ideal for investigating gene expression patterns in nonmodel animal systems. This protocol describes immunostaining for studies of embryogenesis in the tardigrade .
免疫染色是一种用于观察固定组织中蛋白质定位的方法。有许多抗体可识别多种生物体中的特定蛋白质，这使得该方法非常适合用于研究非模式动物系统中的基因表达模式。本方案描述了用于缓步动物胚胎发育研究的免疫染色方法。
