Smith Frank W
Biology Department, University of North Florida, Jacksonville, Florida 32224
Cold Spring Harb Protoc. 2018 Nov 1;2018(11):2018/11/pdb.prot102350. doi: 10.1101/pdb.prot102350.
In situ hybridization is a method for visualizing embryonic gene expression that is amenable to nonmodel systems. Here, an in situ hybridization protocol is presented for the tardigrade This method allows gene expression to be visualized directly and with fluorescence microscopy.
原位杂交是一种可视化胚胎基因表达的方法，适用于非模式生物系统。本文介绍了一种针对缓步动物的原位杂交方案。该方法可直接通过荧光显微镜观察基因表达情况。
