Hatmaker E Anne, Wadl Phillip A, Mantooth Kristie, Scheffler Brian E, Ownley Bonnie H, Trigiano Robert N
Department of Entomology and Plant Pathology, University of Tennessee, 2505 E. J. Chapman Drive, Knoxville, Tennessee 37996-4560 USA.
USDA-ARS Genomics and Bioinformatics Research Unit, 141 Experiment Station Road, P.O. Box 36, Stoneville, Mississippi 38776-0038 USA.
Appl Plant Sci. 2015 Apr 7;3(4). doi: 10.3732/apps.1400123. eCollection 2015 Apr.
We developed microsatellites from Fothergilla ×intermedia to establish loci capable of distinguishing species and cultivars, and to assess genetic diversity for use by ornamental breeders and to transfer within Hamamelidaceae.
We sequenced a small insert genomic library enriched for microsatellites to develop 12 polymorphic microsatellite loci. The number of alleles detected ranged from four to 15 across five genera within Hamamelidaceae. Shannon's information index ranged from 0.07 to 0.14.
These microsatellite loci provide a set of markers to evaluate genetic diversity of natural and cultivated collections and assist ornamental plant breeders for genetic studies of five popular genera of woody ornamental plants.
我们从中间金缕梅（Fothergilla ×intermedia）开发了微卫星，以建立能够区分物种和品种的位点，并评估遗传多样性，供观赏植物育种者使用并在金缕梅科内转移。
我们对富含微卫星的小插入基因组文库进行测序，以开发12个多态性微卫星位点。在金缕梅科的五个属中检测到的等位基因数量从4个到15个不等。香农信息指数范围为0.07至0.14。
这些微卫星位点提供了一组标记，用于评估天然和栽培群体的遗传多样性，并协助观赏植物育种者对五种流行的木本观赏植物属进行遗传研究。
