噬菌体T5的第一步转移DNA注射终止信号
The first-step transfer-DNA injection-stop signal of bacteriophage T5.
作者信息
Heusterspreute M, Ha-Thi V, Tournis-Gamble S, Davison J
出版信息
Gene. 1987;52(2-3):155-64. doi: 10.1016/0378-1119(87)90042-4.
Bacteriophage T5 is different from most phages in that its DNA is injected in two steps during infection. The region containing the injection stop signal (iss) has been cloned and sequenced and found to contain numerous large repeats and inverted repeats which may be part of the iss. The most impressive of these are the 31-bp repeat units (rb) which are present three times in 99 bp. The rb repeats, themselves, contain inverted repeats so that mutually exclusive stem-and-loop structures may potentially form, not only within the repeats, but also between them. Another pair of repeats (21 bp each) contains two sequences resembling DnaA protein-binding sites. The region sequenced also contains one of the T5 site-specific strand interruptions and this was found to lie at the base of a perfect 9-bp palindrome.
噬菌体T5与大多数噬菌体不同，其DNA在感染过程中分两步注入。包含注射终止信号（iss）的区域已被克隆和测序，发现含有许多大的重复序列和反向重复序列，它们可能是iss的一部分。其中最引人注目的是31个碱基对的重复单元（rb），在99个碱基对中出现了三次。rb重复序列本身包含反向重复序列，因此不仅在重复序列内部，而且在它们之间都可能形成互斥的茎环结构。另一对重复序列（各21个碱基对）包含两个类似于DnaA蛋白结合位点的序列。测序区域还包含T5位点特异性链中断之一，发现它位于一个完美的9个碱基对回文序列的底部。
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