Yoon Jaewoo, Yasumoto-Hirose Mina, Kasai Hiroaki
College of Pharmacy, Keimyung University, 1095 Dalgubeoldaero, Dalseo-Gu, Daegu, 42601, Republic of Korea.
Marine Biotechnology Institute, 3-75-1 Heita, Kamaishi, Iwate, 026-0001, Japan.
Arch Microbiol. 2019 May;201(4):499-504. doi: 10.1007/s00203-018-1589-9. Epub 2018 Nov 1.
A novel marine flavobacterial species, designated 3KA7-17, was isolated from the hard coral Acropora formosa D. collected in Japan. The strain was pale-orange pigmented, Gram-stain negative, strictly aerobic, coccus shaped, and non-motile. Preliminary analysis based on the 16S rRNA gene sequence revealed an affiliation with the family Flavobacteriaceae of the phylum Bacteroidetes, and it had the greatest sequence similarity (96.0%) to Seonamhaeicola algicola Gy8. The DNA G + C content was 34.3 mol%. MK-6 was the major menaquinone, with iso-C15:1 H and/or C13:0 3-OH (24.3%), iso-C15:0 (19.5%), iso-C15:0 3-OH (14.2%), and iso-C17:0 3-OH (15.9%) as the main (> 10%) cellular fatty acids. The major polar lipid profile consisted of phosphatidylethanolamine, two unidentified aminolipids, and two unidentified lipids. Based on distinct phylogenetic and phenotypic evidence, the strain represents a novel species of the genus Seonamhaeicola, for which the name Seonamhaeicola acroporae sp. nov. is proposed and the type strain is 3KA7-17 (= KCTC 62713 = NBRC 113410).
从日本采集的美丽鹿角珊瑚中分离出一种新型海洋黄杆菌，命名为3KA7-17。该菌株呈浅橙色，革兰氏染色阴性，严格需氧，球菌状，无运动性。基于16S rRNA基因序列的初步分析表明，它隶属于拟杆菌门黄杆菌科，与海藻南海栖菌Gy8的序列相似性最高（96.0%）。DNA的G+C含量为34.3mol%。MK-6是主要的甲基萘醌，主要（>10%）细胞脂肪酸为异-C15:1H和/或C13:0 3-OH（24.3%）、异-C15:0（19.5%）、异-C15:0 3-OH（14.2%）和异-C17:0 3-OH（15.9%）。主要极性脂质谱由磷脂酰乙醇胺、两种未鉴定的氨基脂质和两种未鉴定的脂质组成。基于独特的系统发育和表型证据，该菌株代表南海栖菌属的一个新物种，为此提出名称南海栖菌美丽鹿角珊瑚新种，模式菌株为3KA7-17（=KCTC 62713=NBRC 113410）。
