Marine College, Shandong University, Weihai, 264209, Shandong, China.
State Key Laboratory of Microbial Technology, Shandong University, Qingdao, 266237, Shandong, China.
Arch Microbiol. 2020 Aug;202(6):1295-1300. doi: 10.1007/s00203-020-01841-y. Epub 2020 Mar 3.
A novel Gram-stain-negative, orange, rod or curved rod, facultatively anaerobic, gliding bacterial strain, designated strain W255, was isolated from Xiaoshi Island, Weihai, China. The strain W255 grows optimally at a 28 °C, pH 7.5, and 3.0% (w/v) NaCl environment. Its colonies are circular, orange, non-transparent, smooth, and approximately 0.2-0.8 mm in diameter, after being cultured for 72 h on marine agar 2216. Cells of the strain W255 are 0.3-0.8 µm wide and 1.0-4.0 µm long, catalase-positive and oxidase-positive. The major cellular fatty acids are iso-C, iso-C G, and iso-C 3-OH. The sole respiratory quinone is MK-6. The major polar lipids include phosphatidylethanolamine, one unidentified amino lipid, one amino glycolipid, and two unidentified lipids (L1 and L2). The phylogenetic analysis based on 16S rRNA gene sequences indicated that strain W255 has the highest similarities with the type strain of the type species of the genus Seonamhaeicola, S. aphaedonensis KCTC 32578 (97.2%), and moderate with 'S. acroporae' KCTC 62713 (96.5%), S. algicola Gy8 (95.4%) and S. marinus B011 (94.5%). The ANI and dDDH values between strain W255 and S. aphaedonensis KCTC 32578 are 86.6% and 31.3%, respectively. The genomic DNA G + C content is 33.5 mol%. On the basis of gene annotation, it was observed that strain W255 have the abilities of nitrate reduction and utilizing various carbon sources, suggesting that this strain might be an important participant in the nitrogen cycle and carbon cycle in the marine environment. Based on the phenotypic, chemotaxonomic and phylogenetic analysis, strain W255 has been considered as a novel species of the genus Seonamhaeicola, for which the name Seonamhaeicola sediminis sp. nov. is proposed. The type strain is W255 (= MCCC 1H00377 = KCTC 72085).
一株新的革兰氏阴性、橙色、杆状或弯曲杆状、兼性厌氧、滑行细菌菌株，被命名为 W255 菌株，从中国威海小石岛分离得到。该菌株 W255 在 28°C、pH7.5 和 3.0%（w/v）NaCl 的环境中生长最佳。在海洋琼脂 2216 上培养 72 小时后，其菌落呈圆形、橙色、不透明、光滑，直径约为 0.2-0.8mm。菌株 W255 的细胞宽 0.3-0.8µm，长 1.0-4.0µm，过氧化氢酶阳性，氧化酶阳性。主要细胞脂肪酸为 iso-C、iso-C G 和 iso-C 3-OH。唯一的呼吸醌是 MK-6。主要极性脂包括磷脂酰乙醇胺、一种未鉴定的氨基酸脂、一种氨基酸糖脂和两种未鉴定的脂（L1 和 L2）。基于 16S rRNA 基因序列的系统发育分析表明，菌株 W255 与属内模式种 Seonamhaeicola aphaedonensis KCTC 32578 的相似性最高（97.2%），与“Seonamhaeicola acroporae”KCTC 62713（96.5%）、S. algicola Gy8（95.4%）和 S. marinus B011（94.5%）的相似性中等。菌株 W255 与 S. aphaedonensis KCTC 32578 的 ANI 和 dDDH 值分别为 86.6%和 31.3%。基因组 DNA G+C 含量为 33.5mol%。根据基因注释，观察到菌株 W255 具有硝酸盐还原和利用各种碳源的能力，这表明该菌株可能是海洋环境中氮循环和碳循环的重要参与者。基于表型、化学分类和系统发育分析，菌株 W255 被认为是 Seonamhaeicola 属的一个新种，因此提出了 Seonamhaeicola sediminis sp. nov. 的新名称。模式菌株为 W255（=MCCC 1H00377=KCTC 72085）。
