构巢曲霉riboB基因座的克隆

Cloning of the riboB locus of Aspergillus nidulans.

作者信息

Oakley C E, Weil C F, Kretz P L, Oakley B R

出版信息

Gene. 1987;53(2-3):293-8. doi: 10.1016/0378-1119(87)90019-9.

Abstract

We have complemented the riboB2 mutation of Aspergillus nidulans by transformation with a plasmid library of wild-type (wt) sequences. We have isolated, by marker rescue from a riboB+ transformant, a plasmid that complements riboB2 efficiently. From this plasmid we have subcloned an A. nidulans sequence that complements riboB2 efficiently and that integrates by homologous recombination at a site closely linked to the riboB locus. We conclude that this sequence contains the wt riboB+ allele.

摘要

我们通过用野生型（wt）序列的质粒文库转化，对构巢曲霉的riboB2突变进行了互补。我们从一个riboB+转化体中通过标记拯救分离出了一个能有效互补riboB2的质粒。从这个质粒中，我们亚克隆了一个构巢曲霉序列，该序列能有效互补riboB2，并通过同源重组整合到与riboB基因座紧密连锁的位点。我们得出结论，这个序列包含野生型riboB+等位基因。