Dowzer C E, Kelly J M
Department of Genetics, University of Adelaide, South Australia, Australia.
Curr Genet. 1989 Jun;15(6):457-9. doi: 10.1007/BF00376804.
The creA gene from A. nidulans has been cloned by complementation of a non-revertable mutant allele using a genomic library and marker rescue techniques. The rescued sequence was subcloned and a 2.3 kb fragment identified which complements several creA mutant alleles. Northern analyses showed that creA encodes a transcript of approximately 1.8 kb in length and that the levels of this transcript varied by up to two fold depending on the carbon source. Transformants containing more than two extra copies of creA grew as wildtype on a range of carbon sources, but there was evidence for tighter carbon catabolite repression.
利用基因组文库和标记拯救技术，通过对构巢曲霉一个不可回复突变等位基因的互补作用，克隆了creA基因。将拯救的序列进行亚克隆，鉴定出一个2.3 kb的片段，它能互补多个creA突变等位基因。Northern分析表明，creA编码一个长度约为1.8 kb的转录本，并且该转录本的水平根据碳源的不同最多可变化两倍。含有两个以上creA额外拷贝的转化体在一系列碳源上生长表现为野生型，但有证据表明碳分解代谢物阻遏作用更强。
