Pluscec J, Owies S
J Assoc Off Anal Chem. 1987 May-Jun;70(3):599-601.
A method is described for the determination of vitamin D3 in a liquid multivitamin preparation by liquid chromatography. Samples are purified on a disposable reverse phase extraction (SPE) column with a mobile phase of methanol-2-propanol (97 + 3) and are analyzed on a Zorbax ODS (5 micron) column with an acetonitrile-2-propanol-water (90 + 8 + 2) solvent system. Vitamin D3 is completely resolved from other interfering compounds within approximately 21 min and is detected with a UV detector at 254 nm. A mean of 98.5% of theory with a coefficient of variation of 3.8% was found for determination of vitamin D3 in a commercial preparation.
描述了一种通过液相色谱法测定液体多种维生素制剂中维生素D3的方法。样品在一次性反相萃取（SPE）柱上用甲醇 - 2 - 丙醇（97 + 3）流动相进行纯化，然后在Zorbax ODS（5微米）柱上用乙腈 - 2 - 丙醇 - 水（90 + 8 + 2）溶剂系统进行分析。维生素D3在约21分钟内与其他干扰化合物完全分离，并用紫外检测器在254 nm处进行检测。在一种商业制剂中测定维生素D3时，发现平均回收率为理论值的98.5%，变异系数为3.8%。
