State Key Laboratory of Material Processing and Die & Mould Technology, School of Materials Science and Engineering, Hubei Key Laboratory of Bioinorganic Chemistry & Materia Medica, School of Chemistry and Chemical Engineering, National Engineering Research Center for Nanomedicine, Department of Biomedical Engineering, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, PR China.
State Key Laboratory of Material Processing and Die & Mould Technology, School of Materials Science and Engineering, Hubei Key Laboratory of Bioinorganic Chemistry & Materia Medica, School of Chemistry and Chemical Engineering, National Engineering Research Center for Nanomedicine, Department of Biomedical Engineering, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, PR China.
Biosens Bioelectron. 2019 Jan 15;124-125:199-204. doi: 10.1016/j.bios.2018.10.009. Epub 2018 Oct 17.
Telomerase and microRNAs (miRNAs) as important biomarkers are closely related to cancers. Simultaneous detection of telomerase activity and miRNAs would be beneficial to improve the specificity and reliability. Here, we establish a telomerase and miRNA-21 (miR-21) simultaneous sensing platform with graphene oxide-based fluorescent aptasensors (GOFA) including graphene oxide (GO), template strand (TS) primer and fluorophore-labeled telomerase/miR-21 oligonucleotides. Owing to π-π stacking interaction, TS primer and telomerase/miR-21 probes would be loaded onto GO, resulting in fluorescence quenching. However, in the presence of the telomerase or miR-21, the double-stranded oligonucleotides would be away from the GO surface attribute to the hybridization between the extended TS primers and telomerase probe as well as miR-21 and miR-21 probe, leading to obvious fluorescence recovery. We found that GOFA could simultaneously detect telomerase activity and miR-21 with low background signal, high sensitivity and simplified operation. Moreover, GOFA could be used for accurately detecting telomerase activity and miRNA in living cells and cancer patient tissue sample. This sensing platform shows great potential in improving the accuracy in clinical diagnosis of cancer.
端粒酶和 microRNAs（miRNAs）作为重要的生物标志物，与癌症密切相关。同时检测端粒酶活性和 miRNAs 将有助于提高特异性和可靠性。在这里，我们建立了一个基于石墨烯氧化物的荧光适体传感器（GOFA）的端粒酶和 miRNA-21（miR-21）同时传感平台，包括石墨烯氧化物（GO）、模板链（TS）引物和荧光标记的端粒酶/miR-21 寡核苷酸。由于 π-π 堆积相互作用，TS 引物和端粒酶/miR-21 探针将被加载到 GO 上，导致荧光猝灭。然而，在存在端粒酶或 miR-21 的情况下，双链寡核苷酸会远离 GO 表面，这是由于延伸的 TS 引物与端粒酶探针以及 miR-21 与 miR-21 探针之间的杂交所致，导致明显的荧光恢复。我们发现，GOFA 可以在低背景信号、高灵敏度和简化操作的情况下同时检测端粒酶活性和 miR-21。此外，GOFA 可用于准确检测活细胞和癌症患者组织样本中的端粒酶活性和 miRNA。该传感平台在提高癌症临床诊断的准确性方面具有很大的潜力。
