卷荚相思（DC.）Guill. & Perr.根增强雄性 Wistar 大鼠的生殖行为和性功能：生化和药理学机制。

Acridocarpus Smeathmannii (DC.) Guill. & Perr. Root enhanced reproductive behavior and sexual function in male wistar rats: Biochemical and pharmacological mechanisms.

机构信息

Department of Pharmacology, Therapeutics & Toxicology, College of Medicine, University of Lagos, P.M.B 12003 Idi-Araba, Lagos, Nigeria.

出版信息

J Ethnopharmacol. 2019 Feb 10;230:95-108. doi: 10.1016/j.jep.2018.10.024. Epub 2018 Oct 31.

DOI:10.1016/j.jep.2018.10.024

PMID:30389468

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

Novel therapeutic opportunities from medicinal agents continue to arouse scientific interest in recent times. Still, there is a dearth of information as regards experimental evidence generated from medicinal plants that would yield pharmacological agents for the treatment of erectile dysfunction. Acridocarpus Smeathmannii (DC.) Guill. & Perr. Root (ASR) has a long history as an aphrodisiac in African traditional medicine. Thus, this study investigated the reproductive potentials and associated biochemical mechanisms of its hydroethanolic extract (HEASR) in male Wistar rats. Also, the bioactive compounds were identified.

MATERIALS AND METHODS

Fifty-four male albino rats (180 ± 20 g) were divided into nine groups of six rats/group. Control, group 1 received normal saline (10 mL/kg). Groups 2-6 rats were administered sildenafil (1.43 mg/kg/day), mesterolone (0.36 mg/kg/day), doxazocin (0.03 mg/kg/day), HEASR1 (50 mg/kg/day) and HEASR2 (200 mg/kg/day) respectively. Others received co-administration of HEASR2 with standard drugs. Treatment lasted for 28 days via oral gavage.

RESULTS

An acute oral toxicity of HEASR up to 2 g/kg produced no mortality in mice p.o. while the median lethal dose was estimated to be 810 mg/kg i.p. HEASR2 administration or in combination with sildenafil, mesterolone and doxazocin increased mounting frequencies on day 28 by 77.44%, 122.65%, 148.5% and 93.88% and sperm counts by 38.29%, 55.21%, 42.48%, and 48.98% respectively in treated rats. HEASR2 + sildenafil elevated testosterone and follicle stimulating hormone levels by 36.33% and 24.55% while HEASR2 + doxazocin elevated luteinizing hormone levels by 97.44% in rats. HEASR modulated prostate-specific antigen and malondialdehyde levels respectively. Reduced glutathione, superoxide dismutase, and catalase activities were raised in five selected organs. Serum nitric oxide but not cyclooxygenase-2 or tumor necrosis factor-α levels was moderately improved in rats.

CONCLUSION

Overall, the results obtained demonstrated the potential of HEASR as a male reproductive enhancer, thus justify its folklore applications. Further, octadecanoic acid ethyl ester was the most abundant bioactive component present.

摘要

民族药理学相关性

近年来，新型治疗药物不断引起科学界对药用制剂的兴趣。然而，关于药用植物产生的实验证据，仍然缺乏能够产生治疗勃起功能障碍的药理学制剂的信息。非洲传统医学中，Acridocarpus Smeathmannii (DC.) Guill. & Perr. Root（ASR）作为春药已有很长的历史。因此，本研究调查了其水乙醇提取物（HEASR）在雄性 Wistar 大鼠中的生殖潜力和相关生化机制。此外，还鉴定了生物活性化合物。

材料和方法

54 只雄性白化大鼠（180±20g）分为 9 组，每组 6 只。对照组，第 1 组给予生理盐水（10ml/kg）。第 2-6 组大鼠分别给予西地那非（1.43mg/kg/天）、美睾酮（0.36mg/kg/天）、多沙唑嗪（0.03mg/kg/天）、HEASR1（50mg/kg/天）和 HEASR2（200mg/kg/天）。其他组接受 HEASR2 与标准药物联合给药。通过口服灌胃治疗 28 天。

结果

急性口服毒性试验表明，HEASR 高达 2g/kg 时，在小鼠中未产生死亡率，而估计半数致死量为 810mg/kg ip。HEASR2 给药或与西地那非、美睾酮和多沙唑嗪联合给药可使治疗大鼠第 28 天的交配频率分别增加 77.44%、122.65%、148.5%和 93.88%，精子计数分别增加 38.29%、55.21%、42.48%和 48.98%。HEASR2+西地那非可使大鼠的睾酮和促卵泡激素水平分别升高 36.33%和 24.55%，而 HEASR2+多沙唑嗪可使大鼠的黄体生成素水平升高 97.44%。HEASR 调节前列腺特异性抗原和丙二醛水平。五种选定器官中的还原型谷胱甘肽、超氧化物歧化酶和过氧化氢酶活性升高。大鼠血清一氧化氮但环氧化酶-2 或肿瘤坏死因子-α水平仅适度改善。