间接免疫荧光法与微量中和法检测脊髓灰质炎病毒抗体的比较
Comparison between indirect immunofluorescence and microneutralization for detection of antibodies to polioviruses.
作者信息
Pettit C, Minnich L L, Shehab Z M, Ray C G
出版信息
J Clin Microbiol. 1987 Jul;25(7):1325-6. doi: 10.1128/jcm.25.7.1325-1326.1987.
Indirect immunofluorescence and microneutralization methods for the detection of antibodies to poliovirus serotypes 1, 2, and 3 were compared. Of the 41 sera tested for poliovirus type 1 antibody, 40% were in complete agreement, 55% differed by one dilution, and 5% differed by two dilutions. For poliovirus type 2, 37 sera were tested; 56% completely agreed, and 44% differed by only one dilution. For poliovirus type 3, complete agreement occurred in 59% of 33 sera, while 41% differed by one dilution. No false-negative results were obtained. These findings suggest that indirect immunofluorescence for poliovirus types 1, 2, and 3 is as sensitive as the microneutralization method and could represent a less cumbersome alternative.
对检测脊髓灰质炎病毒1型、2型和3型抗体的间接免疫荧光法和微量中和法进行了比较。在检测1型脊髓灰质炎病毒抗体的41份血清中，40%完全一致，55%相差一个稀释度，5%相差两个稀释度。对于2型脊髓灰质炎病毒，检测了37份血清；56%完全一致，44%仅相差一个稀释度。对于3型脊髓灰质炎病毒，33份血清中有59%完全一致，41%相差一个稀释度。未获得假阴性结果。这些发现表明，用于1型、2型和3型脊髓灰质炎病毒的间接免疫荧光法与微量中和法一样敏感，可能是一种不那么繁琐的替代方法。
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