Egorov N S, Baranova I P, Kozlova Iu I
Antibiotiki. 1978 Oct;23(10):872-4.
The problem of microbial cell immobilization at present attracts the ever increasing attention of the scientists, since such organisms may be the source of various enzymes. Production of nizin by the immobilized cells of Str. lactis was studied. It was found that the cells of Str. lactis incorporated into polyacrylamide gel produced nizit on definite media. Still, the amount of the antibiotic was 2-3 times lower than in case of using free cells. The effect of a number of factors on the process of immobilization was studied and the influence of some factors, such as temperature, pH, aeration on nizin synthesis by the immobilized cells of the streptococcus was elucidated. Optimal conditions for nizin biosynthesis by the immobilized cells of Str. lactis were developed.
目前，微生物细胞固定化问题日益引起科学家们的关注，因为这类生物体可能是各种酶的来源。研究了乳酸链球菌固定化细胞生产乳酸链球菌素的情况。发现包埋在聚丙烯酰胺凝胶中的乳酸链球菌细胞在特定培养基上能产生乳酸链球菌素。然而，抗生素的产量比使用游离细胞时低2至3倍。研究了多种因素对固定化过程的影响，并阐明了一些因素，如温度、pH值、通气对链球菌固定化细胞合成乳酸链球菌素的影响。确定了乳酸链球菌固定化细胞生物合成乳酸链球菌素的最佳条件。
