Heberling R L, Kalter S S
Lab Anim Sci. 1987 Jun;37(3):304-8.
An enzyme-immunoassay performed with Herpesvirus simiae (B virus) and H. simplex antigens inactivated with a psoralen derivative and long-wavelength ultraviolet light irradiation is described. Although B virus is a known human pathogen requiring extreme care in its handling, the use of inactivated antigens in the test allows its performance without biosafety containment. The test utilizes nitrocellulose sheets dotted with antigen for the assay of antibody against B virus in nonhuman primate sera. Antigen-antibody complexes are detected visually as red dots by the use of enzyme-conjugated antiglobulin second antibody and a substrate that produces an insoluble product. The test is more rapid, sensitive and specific than the serum neutralization test it is intended to replace. Of 150 macaque monkey sera tested, 83 were negative by the enzyme and neutralization tests, 56 were positive by both tests and 11 were positive by enzyme-assay but negative by neutralization. Positive sera reacted with both simian and human viral antigens in the enzyme assay but with greater intensity against B virus. Absorption with H. simplex removes reactivity with this virus without reducing the B virus response.
本文描述了一种酶免疫测定法，该方法使用经补骨脂素衍生物和长波长紫外线照射灭活的猴疱疹病毒（B病毒）和单纯疱疹病毒抗原。尽管B病毒是一种已知的人类病原体，处理时需要格外小心，但在检测中使用灭活抗原可在无生物安全防护的情况下进行检测。该检测利用点有抗原的硝酸纤维素膜来检测非人灵长类动物血清中抗B病毒的抗体。通过使用酶联抗球蛋白二抗和产生不溶性产物的底物，抗原-抗体复合物可直观地检测为红色斑点。该检测比其旨在替代的血清中和试验更快、更灵敏且更具特异性。在检测的150份猕猴血清中，83份经酶法和中和试验均为阴性，56份两项试验均为阳性，11份酶法检测为阳性但中和试验为阴性。阳性血清在酶法检测中与猿猴和人类病毒抗原均有反应，但对B病毒的反应更强。用单纯疱疹病毒进行吸收可消除与该病毒的反应性，而不降低对B病毒的反应。
