Kalter S S, Hutt R, Guajardo J E, Heberling R L, Lester T L, Pleasant L C
Dev Biol Stand. 1978;41:235-40.
The presence of herpesviruses in various animal tissues used for vaccine production is of importance, inasmuch as these viruses may go unrecognized, residing in latent form. Of greatest concern is Herpesvirus simiae (B virus) present in the majority of macaca species, one of the more extensively used laboratory nonhuman primates. The close antigenic relationship of B virus to human herpes strains (H. hominis) and SA8 (a baboon herpesvirus) makes serologic differentiation extremely difficult, but necessary. Complicating the differential diagnosis is the frequent infection of simian species with human strains. The present study clearly shows that many animal sera negative to herpes simplex will be negative to B virus in the neutralization test. However, a sufficient number of sera will be negative to simplex, but positive to B virus, indicating that the assumed one-way cross is invalid. Also, if antibody to simplex is present, and this antibody is present in most adult macaques, then differentiation requires testing with B virus. At least 50% of these positive sera may appear to be negative for B virus if only tested with H. hominis. Approximately 25% of the remaining positive sera will have higher titers to B virus than to herpes simplex and 25% will have the same titers to both viruses. Further, complement is required by many of the test sera to detect the presence of antibodies to B virus.
用于疫苗生产的各种动物组织中存在疱疹病毒至关重要，因为这些病毒可能以潜伏形式存在而未被识别。最令人担忧的是猕猴疱疹病毒（B病毒），它存在于大多数猕猴属物种中，而猕猴是使用较为广泛的实验室非人灵长类动物之一。B病毒与人类疱疹病毒株（人疱疹病毒）和SA8（一种狒狒疱疹病毒）的抗原关系密切，这使得血清学鉴别极为困难，但又十分必要。使鉴别诊断复杂化的是猿猴物种经常感染人类病毒株。本研究清楚地表明，许多对单纯疱疹病毒呈阴性的动物血清在中和试验中对B病毒也呈阴性。然而，有相当数量的血清对单纯疱疹病毒呈阴性，但对B病毒呈阳性，这表明假定的单向交叉是无效的。此外，如果存在针对单纯疱疹病毒的抗体（大多数成年猕猴体内都有这种抗体），那么进行鉴别就需要用B病毒进行检测。如果仅用人疱疹病毒进行检测，这些阳性血清中至少50%对B病毒可能呈阴性。其余阳性血清中约25%对B病毒的滴度高于对单纯疱疹病毒的滴度，25%对两种病毒的滴度相同。此外，许多检测血清需要补体来检测B病毒抗体的存在。
