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斑点免疫结合试验与酶联免疫吸附试验用于快速、特异性检测人或猴获得性免疫缺陷综合征诱导产生的逆转录病毒抗体的比较

Dot immunobinding assay compared with enzyme-linked immunosorbent assay for rapid and specific detection of retrovirus antibody induced by human or simian acquired immunodeficiency syndrome.

作者信息

Heberling R L, Kalter S S, Marx P A, Lowry J K, Rodriguez A R

机构信息

Department of Virology and Immunology, Southwest Foundation for Biomedical Research, San Antonio, Texas 78284.

出版信息

J Clin Microbiol. 1988 Apr;26(4):765-7. doi: 10.1128/jcm.26.4.765-767.1988.

Abstract

A rapid, specific, and sensitive modification of the dot immunobinding assay was compared with the standard enzyme immunoassay as a screening procedure for the detection of antibody in human or simian acquired immunodeficiency syndrome. Comparative testing with the available enzyme immunoassay procedures, either in commercial kit form or as provided by diagnostic laboratories, indicated excellent correlation. Ease of operation and cost are key features of the dot immunobinding assay procedure.

摘要

作为检测人类或猿类获得性免疫缺陷综合征中抗体的一种筛查方法,将斑点免疫结合试验的一种快速、特异且灵敏的改良方法与标准酶免疫测定法进行了比较。与现有的酶免疫测定方法(无论是商业试剂盒形式还是诊断实验室提供的方法)进行的对比测试表明具有良好的相关性。操作简便和成本低是斑点免疫结合试验方法的关键特性。

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