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斑点免疫结合试验与酶联免疫吸附试验用于快速、特异性检测人或猴获得性免疫缺陷综合征诱导产生的逆转录病毒抗体的比较

Dot immunobinding assay compared with enzyme-linked immunosorbent assay for rapid and specific detection of retrovirus antibody induced by human or simian acquired immunodeficiency syndrome.

作者信息

Heberling R L, Kalter S S, Marx P A, Lowry J K, Rodriguez A R

机构信息

Department of Virology and Immunology, Southwest Foundation for Biomedical Research, San Antonio, Texas 78284.

出版信息

J Clin Microbiol. 1988 Apr;26(4):765-7. doi: 10.1128/jcm.26.4.765-767.1988.

DOI:10.1128/jcm.26.4.765-767.1988
PMID:2835392
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC266443/
Abstract

A rapid, specific, and sensitive modification of the dot immunobinding assay was compared with the standard enzyme immunoassay as a screening procedure for the detection of antibody in human or simian acquired immunodeficiency syndrome. Comparative testing with the available enzyme immunoassay procedures, either in commercial kit form or as provided by diagnostic laboratories, indicated excellent correlation. Ease of operation and cost are key features of the dot immunobinding assay procedure.

摘要

作为检测人类或猿类获得性免疫缺陷综合征中抗体的一种筛查方法，将斑点免疫结合试验的一种快速、特异且灵敏的改良方法与标准酶免疫测定法进行了比较。与现有的酶免疫测定方法（无论是商业试剂盒形式还是诊断实验室提供的方法）进行的对比测试表明具有良好的相关性。操作简便和成本低是斑点免疫结合试验方法的关键特性。

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Dot immunobinding assay compared with enzyme-linked immunosorbent assay for rapid and specific detection of retrovirus antibody induced by human or simian acquired immunodeficiency syndrome.斑点免疫结合试验与酶联免疫吸附试验用于快速、特异性检测人或猴获得性免疫缺陷综合征诱导产生的逆转录病毒抗体的比较
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2
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本文引用的文献

1
Simian AIDS: isolation of a type D retrovirus and transmission of the disease.猿猴艾滋病：一种D型逆转录病毒的分离及该疾病的传播
Science. 1984 Mar 9;223(4640):1083-6. doi: 10.1126/science.6695196.
2
A new type D retrovirus isolated from macaques with an immunodeficiency syndrome.从患有免疫缺陷综合征的猕猴中分离出的一种新型D型逆转录病毒。
Science. 1984 Feb 10;223(4636):602-5. doi: 10.1126/science.6695172.
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Simian AIDS--evidence for a retroviral etiology.猿猴艾滋病——逆转录病毒病因学的证据。
Hematol Oncol. 1984 Jul-Sep;2(3):259-68. doi: 10.1002/hon.2900020305.
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Discussion paper: cross-reactivity of primate immunoglobulins.讨论文件：灵长类免疫球蛋白的交叉反应性
Ann N Y Acad Sci. 1969 Jul 3;162(1):195-201. doi: 10.1111/j.1749-6632.1969.tb56363.x.
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Simian models of acquired immunodeficiency syndrome (AIDS): a review.获得性免疫缺陷综合征（艾滋病）的猿猴模型综述
Vet Pathol. 1986 Jul;23(4):345-53. doi: 10.1177/030098588602300401.
6
Serodiagnosis of rabies by dot immunobinding assay.用斑点免疫结合试验进行狂犬病的血清学诊断。
J Clin Microbiol. 1987 Jul;25(7):1262-4. doi: 10.1128/jcm.25.7.1262-1264.1987.
7
Dot enzyme immunoassay. A simple, cheap and stable test for antibody to human immunodeficiency virus (HIV).斑点酶免疫测定法。一种用于检测人类免疫缺陷病毒（HIV）抗体的简单、廉价且稳定的检测方法。
J Immunol Methods. 1987 May 20;99(2):191-4. doi: 10.1016/0022-1759(87)90126-8.
8
Isolation of T-cell tropic HTLV-III-like retrovirus from macaques.从猕猴中分离出嗜T细胞的HTLV-III样逆转录病毒。
Science. 1985 Jun 7;228(4704):1201-4. doi: 10.1126/science.3159089.
9
A dot-immunobinding assay on nitrocellulose with psoralen inactivated Herpesvirus simiae (B virus).用补骨脂素灭活的猴疱疹病毒（B病毒）在硝酸纤维素膜上进行斑点免疫结合试验。
Lab Anim Sci. 1987 Jun;37(3):304-8.
10
The causes of false-positives encountered during the screening of old-world primates for antibodies to human and simian retroviruses by ELISA.在通过酶联免疫吸附测定法（ELISA）对旧世界灵长类动物进行人类和猿猴逆转录病毒抗体筛查期间遇到假阳性结果的原因。
J Virol Methods. 1986 Nov;14(3-4):213-28. doi: 10.1016/0166-0934(86)90023-6.