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通过位于“愚钝”基因座的一个代表性成员鉴定出的果蝇多聚脱氧腺苷酸化类转座元件的一个新家族。

A new family of the poly-deoxyadenylated class of Drosophila transposable elements identified by a representative member at the dunce locus.

作者信息

Pittler S J, Davis R L

出版信息

Mol Gen Genet. 1987 Jun;208(1-2):325-8. doi: 10.1007/BF00330460.

Abstract

Two transposable elements have been identified at the dunce locus in chromosomes recovered after a premeiotic and interchromosomal conversion event occurred at this gene. One is approximately 8.2 kb and is inserted near the 5' end of the gene. This element was identified by sequence analysis as a member of the B104 (roo) family of copia-like transposable elements. The second resides near the 3' end of the gene and represents a new family of the class of poly-deoxyadenylated [poly(dA)] transposable elements. It is 0.38 kb in length and has one terminus consisting of a stretch of 29 deoxyadenosine residues with a polyadenylation site like those found in mRNA molecules, located about 20 bp away from the poly(dA) stretch. Fourteen base pairs of genome DNA is duplicated at the target site of this element.

摘要

在该基因发生减数分裂前和染色体间转换事件后回收的染色体中,已在“愚钝”基因座处鉴定出两个转座元件。其中一个约8.2 kb,插入在该基因5'端附近。通过序列分析确定该元件是类copia转座元件B104(roo)家族的成员。第二个位于该基因3'端附近,代表聚脱氧腺苷酸化[poly(dA)]转座元件类的一个新家族。它长度为0.38 kb,有一个末端由29个脱氧腺苷残基组成的片段,带有一个聚腺苷酸化位点,类似于在mRNA分子中发现的那些,位于离poly(dA)片段约20 bp处。该元件的靶位点处有14个碱基对的基因组DNA发生了重复。

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