Wang Chengqiang, Li Hongxing, Xu Lili, Shen Yu, Hou Jin, Bao Xiaoming
Shandong Key Laboratory of Agricultural Microbiology, College of Life Sciences, Shandong Agricultural University, Tai'an 271018, Shandong, China.
The State Key laboratory of Microbial Technology, College of Life Sciences, Shandong University, Jinan 250100, Shandong, China.
Sheng Wu Gong Cheng Xue Bao. 2018 Oct 25;34(10):1543-1555. doi: 10.13345/j.cjb.180031.
One of the requirements for increasing the economic profitability on the large-scale production of second-generation ethanol and other bio-chemicals using lignocellulose biomass as raw materials is efficient hexose and pentose utilization. Saccharomyces cerevisiae, the traditional ethanol producer, is an attractive chassis cell due to its robustness towards harsh environmental conditions and inherent advantages. But S. cerevisiae cannot utilize pentose. The precision construction of suitable strains for second-generation bio-ethanol production has been taken for more than three decades based on the principle of metabolic engineering and synthetic biology. The resulting strains have improved significantly co-fermentation of glucose and xylose. Recently, much attentions have been focused on sugar transport, which is one of the limiting but formerly ignored step for ethanol production from both glucose and xylose, to get the desired state that different sugars could efficiently delivered by their individual specific transporters. In this paper, the progress on sugar transporters of S. cerevisiae was reviewed, and the research status of xylose and/or L-arabinose metabolic engineering in S. cerevisiae were also presented.
使用木质纤维素生物质作为原料大规模生产第二代乙醇和其他生物化学品时，提高经济盈利能力的要求之一是高效利用己糖和戊糖。传统的乙醇生产菌酿酒酵母，由于其对恶劣环境条件的耐受性和固有优势，是一种有吸引力的底盘细胞。但是酿酒酵母不能利用戊糖。基于代谢工程和合成生物学原理，为第二代生物乙醇生产构建合适菌株的精确工程已经进行了三十多年。由此产生的菌株在葡萄糖和木糖的共发酵方面有了显著改善。最近，人们的注意力集中在糖转运上，糖转运是从葡萄糖和木糖生产乙醇的一个限制因素，但以前被忽视了，目的是达到不同糖类能够通过各自特定的转运蛋白有效转运的理想状态。本文综述了酿酒酵母糖转运蛋白的研究进展，并介绍了酿酒酵母木糖和/或L-阿拉伯糖代谢工程的研究现状。
