[在大鼠宫腔粘连模型中对用PKH26标记的人羊膜间充质干细胞进行体内追踪]
[In vivo tracing of human amniotic mesenchymal stem cells labeled with PKH26 in rat intrauterine adhesions model].
作者信息
Yang Yuan, Mao Yanhua, Wang Jia, Sun Congcong, Zhang Yingfeng, Chen Xinpei
机构信息
Obstetrics and Gynecology Departement, the University-Town Hospital, Chongqing Medical University, Chongqing 401331, China.
出版信息
Sheng Wu Gong Cheng Xue Bao. 2018 Oct 25;34(10):1660-1667. doi: 10.13345/j.cjb.180018.
To observe the migration of human amniotic mesenchymal stem cells (hAMSCs) labeled with PKH26 in the endometrium of rats intrauterine adhesion. hAMSCs were isolated, identified and labeled with PKH26 to detect the biological characteristics of the cells. Rat intrauterine adhesion models were established using mechanical and infective method and PKH26-labeled hAMSCs were transplanted through the tail vein. The distribution of PKH26 labeled hAMSCs in the endometrium of rats were observed with the fluorescence confocal microscope. The results showed that PKH26 stain had no significant effect on cell activity, cycle, apoptosis and so on. PKH26-labeled positive cells were mainly distributed in injured endometrium of rats. It shows that the PKH26 labeling technique is a safe and effective method for tracing the human amniotic mesenchymal stem cells in the treatment of intrauterine adhesions.
观察携带PKH26标记的人羊膜间充质干细胞（hAMSCs）在大鼠宫腔粘连子宫内膜中的迁移情况。分离、鉴定hAMSCs并用PKH26进行标记以检测细胞生物学特性。采用机械和感染方法建立大鼠宫腔粘连模型，经尾静脉移植PKH26标记的hAMSCs。用荧光共聚焦显微镜观察PKH26标记的hAMSCs在大鼠子宫内膜中的分布。结果显示，PKH26染色对细胞活性、周期、凋亡等无显著影响。PKH26标记的阳性细胞主要分布于大鼠受损的子宫内膜。表明PKH26标记技术是追踪人羊膜间充质干细胞治疗宫腔粘连的一种安全有效的方法。
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