Hirumi H, Doyle J J, Hirumi K
Bull World Health Organ. 1977;55(2-3):405-9.
Animal-infective forms of Trypanosoma brucei (Strain 427) were successfully propagated in HEPES-buffered RPMI 1640 medium in the presence of bovine fibroblast-like cells for over 310 days. The organisms grown in this system were morphologically identical to the long slender bloodstream forms, retained their infectivity for mammalian hosts, and displayed variant-antigen on their surface. Technical details for establishing such bloodstream form cultures are described in the present paper.
布氏锥虫（427株）的动物感染性形态在含有牛成纤维细胞样细胞的HEPES缓冲RPMI 1640培养基中成功繁殖了310多天。在该系统中生长的生物体在形态上与长细的血流形式相同，保留了对哺乳动物宿主的感染性，并在其表面显示出变异抗原。本文描述了建立这种血流形式培养物的技术细节。
