School of Chemistry and Chemical Engineering, Shandong University of Technology, Zibo 255049, PR China; Key Laboratory of Interfacial Reaction & Sensing Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
School of Chemistry and Chemical Engineering, Shandong University of Technology, Zibo 255049, PR China.
Biosens Bioelectron. 2019 Feb 1;126:108-114. doi: 10.1016/j.bios.2018.10.017. Epub 2018 Oct 13.
Here, a novel HO-based electrochemical immunosensor utilizing Pd nanoparticles functionalized three-dimensional wrinkly amorphous MoS composites (Pd NPs@3D MoS) as the platform was developed for the determination of insulin. In this work, Pd NPs@3D MoS prepared in the presence of CTAB possessed an excellent catalytic activity for the reduction of HO. Furthermore, Pd NPs@3D MoS with favorable biological compatibility can conjugate a great many antibodies to capture insulin. Attributed to the excellent property, electrochemical signals could be greatly amplified, contributing to improving detection sensitivity. Especially, SEM, TEM, and XPS information further confirmed nanomaterial's surface morphology and amorphous structure. Under the optimal conditions, the proposed immunosensor exhibited a sensitively linear relation with logarithmic insulin concentrations from 0.01 to 100 ng/mL with a low detection limit of 3.0 pg/mL (S/N = 3). Characterized by good reproducibility, specificity, and stability, the fabricated immunosensor may blaze a path for insulin detection in a real sample.
在这里，我们开发了一种基于 HO 的新型电化学免疫传感器，该传感器利用 Pd 纳米粒子功能化的三维皱状非晶态 MoS 复合材料（Pd NPs@3D MoS）作为平台，用于胰岛素的测定。在这项工作中，在 CTAB 存在下制备的 Pd NPs@3D MoS 对 HO 的还原具有优异的催化活性。此外，具有良好生物相容性的 Pd NPs@3D MoS 可以结合大量抗体来捕获胰岛素。由于其优异的性能，电化学信号可以大大放大，有助于提高检测灵敏度。特别是 SEM、TEM 和 XPS 信息进一步证实了纳米材料的表面形态和非晶结构。在最佳条件下，所提出的免疫传感器在 0.01 至 100ng/mL 的对数胰岛素浓度范围内表现出灵敏的线性关系，检测限低至 3.0pg/mL（S/N=3）。所制备的免疫传感器具有良好的重现性、特异性和稳定性，可为实际样品中的胰岛素检测开辟道路。
