Key Laboratory of Interfacial Reaction & Sensing Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, PR China.
Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science, MOE; Shandong Key Laboratory of Biochemical Analysis; Key Laboratory of Analytical Chemistry for Life Science in Universities of Shandong; College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, PR China.
Biosens Bioelectron. 2019 May 1;132:97-104. doi: 10.1016/j.bios.2019.02.055. Epub 2019 Mar 4.
A sandwich-type electrochemical immunosensor for prostate-specific antigen (PSA) detection was constructed. In this sensing platform, palladium nanoparticles (Pd NPs) loaded electroactive amino-zeolitic imidazolate framework-67 (Pd/NH-ZIF-67) nanocomposite is utilized as a novel redox mediator. Pd NPs, as the labeling signal molecule, owns good biocompatibility and excellent catalytic performance. Meanwhile, the electroactive ZIF-67 with lamellar structures serves not only as a carrier of Pd NPs but also as a synergistic catalyst of palladium to decompose HO. Amperometric method is used to determine the current signal generated on the electrode surface, which is conducive to achieve the quantitative determination of PSA. Under optimum conditions, the proposed immunosensor shows a wide detection range from 100 fg mL to 50 ng mL with a low detection limit of 0.03 pg mL, featuring favorable selectivity, repeatability and stability. This proposed immunosensor is expected to be a powerful tool for realizing quantitative detection of PSA and other biomarkers in actural sample analysis.
一种用于检测前列腺特异性抗原（PSA）的三明治型电化学免疫传感器被构建。在这个传感平台中，负载了电活性氨基沸石咪唑酯骨架-67（Pd/NH-ZIF-67）纳米复合材料的钯纳米粒子（Pd NPs）被用作一种新型的氧化还原介体。Pd NPs 作为标记信号分子，具有良好的生物相容性和优异的催化性能。同时，具有层状结构的电活性 ZIF-67 不仅作为 Pd NPs 的载体，而且作为钯协同催化剂来分解 HO。安培法用于检测电极表面产生的电流信号，这有利于实现 PSA 的定量测定。在最佳条件下，所提出的免疫传感器具有从 100 fg mL 到 50 ng mL 的宽检测范围和 0.03 pg mL 的低检测限，表现出良好的选择性、重复性和稳定性。该免疫传感器有望成为实现实际样品分析中 PSA 和其他生物标志物定量检测的有力工具。
