Hughes C S, Jones R C, Gaskell R M, Jordan F T, Bradbury J M
Res Vet Sci. 1987 May;42(3):407-10.
Two separate groups of nine-week-old specific pathogen free cockerels maintained in isolation were infected with a field strain of infectious laryngotracheitis (ILT) virus, either by intratracheal or combined intranasal and supraconjunctival inoculation. Birds were monitored for virus shedding from five sites on alternate days during the acute phase and three times weekly until week 17. They were then treated with cyclophosphamide on three consecutive days and thereafter swabbed daily. During the acute phase clinical signs were observed and virus was recovered from ocular and nasal sites for up to six to eight days. Initially after the acute phase no virus could be detected. However, from the seventh week after infection intermittent, apparently spontaneous shedding was detected in four of five birds in each group. There was no clear effect of cyclophosphamide treatment on re-excretion patterns, possibly because of the high levels of virus shedding already occurring. Thus, a carrier state for ILT virus has been demonstrated experimentally in live clinically recovered birds.
将两组单独饲养的9周龄无特定病原体公鸡隔离起来，通过气管内接种或鼻内联合结膜上接种，用传染性喉气管炎（ILT）病毒的一株野毒株进行感染。在急性期，每隔一天对鸡的五个部位进行病毒排泄监测，直到第17周每周监测三次。然后连续三天用环磷酰胺对它们进行治疗，之后每天进行拭子取样。在急性期观察临床症状，在眼部和鼻部部位可检出病毒长达6至8天。急性期过后最初检测不到病毒。然而，在感染后第七周，每组五只鸡中有四只检测到间歇性、明显是自发的病毒排泄。环磷酰胺治疗对病毒再次排泄模式没有明显影响，可能是因为已经出现了高水平的病毒排泄。因此，在临床上康复的活鸡中通过实验证明了ILT病毒的携带状态。
