Izuchi T, Hasegawa A, Miyamoto T
Avian Dis. 1983 Oct-Dec;27(4):918-26.
The biological properties of tissue-culture-modified strain C7 of infectious laryngotracheitis (ILT) virus were studied. Strain C7 formed well-defined small white pocks that were distinguishable from those formed by the original strain when it was inoculated onto the chorioallantoic membrane of embryonating chicken eggs (pock marker). Strain C7 grew in chicken kidney cell cultures and embryonating chicken eggs better than the original strain. When inoculated intratracheally into chickens, strain C7 propagated primarily in the larynx, trachea, and lung, but the virus was unrecoverable after 6 days postinoculation. No contact infection was demonstrated among chickens. These biological characteristics of strain C7 were stable through five consecutive back-passages in susceptible chickens. In chickens vaccinated ocularly at 14 days of age or older, strain C7 gave good protection against challenge with the virulent strain of ILT virus. These results indicate that strain C7 may be an adequate candidate for a live virus vaccine against ILT.
对传染性喉气管炎（ILT）病毒的组织培养修饰株C7的生物学特性进行了研究。当将C7株接种到鸡胚的绒毛尿囊膜上时（痘疱标记），它形成界限清晰的小白痘疱，与原始毒株形成的痘疱不同。C7株在鸡肾细胞培养物和鸡胚中的生长情况优于原始毒株。当经气管内接种到鸡体内时，C7株主要在喉、气管和肺中增殖，但接种后6天病毒就无法再分离到。未在鸡之间证明有接触感染。通过在易感鸡中连续传代五次，C7株的这些生物学特性保持稳定。在14日龄及以上经眼接种疫苗的鸡中，C7株对强毒ILT病毒攻击具有良好的保护作用。这些结果表明，C7株可能是一种合适的抗ILT活病毒疫苗候选株。
