Lee Jae-Min, Kim Tae-Woon, Park Sang-Seo, Han Jin-Hee, Shin Mal-Soon, Lim Baek-Vin, Kim Sang-Hoon, Baek Seung-Soo, Cho Young Sam, Kim Khae Hawn
Department of Physiology, College of Medicine, Kyung Hee University, Seoul, Korea.
Department of Anesthesiology and Pain Medicine, Kyung Hee Medical Center, College of Medicine, Kyung Hee University, Seoul, Korea.
Int Neurourol J. 2018 Oct;22(Suppl 3):S147-155. doi: 10.5213/inj.1836226.113. Epub 2018 Oct 31.
Rotenone is the most widely used neurotoxin for the making Parkinson disease (PD) animal model. The neurodegenerative disorder PD shows symptoms, such as slowness of movements, tremor at resting, rigidity, disturbance of gait, and instability of posture. We investigated whether treadmill running improves motor ability using rotenone-caused PD rats. The effect of treadmill running on PD was also assessed in relation with apoptosis of cerebellar Purkinje cells.
Treadmill running was applied to the rats in the exercise groups for 30 minutes once a day for 4 weeks, starting 4 weeks after birth. We used rota-rod test for the determination of motor coordination and balance. In this experiment, terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) staining, immunohistochemistry for calbindin, glial fibrillary acidic protein (GFAP), Iba-1, and western blot analysis for Bax and Bcl-2 were performed.
Treadmill running enhanced motor balance and coordination by preventing the loss of Purkinje cells in the cerebellar vermis. Treadmill running suppressed PD-induced expression of GFAP-positive reactive astrocytes and Iba-1-positive microglia, showing that treadmill running suppressed reactive astrogliosis and microglia activation. Treadmill running suppressed TUNEL-positive cell number and Bax expression and enhanced Bcl-2 expression, demonstrating that treadmill running inhibited the progress of apoptosis in the cerebellum of rotenone-induced PD rats.
Treadmill running improved motor ability of the rotenone-induced PD rats by inhibiting apoptosis in the cerebellum. Apoptosis suppressing effect of treadmill running on rotenone-induced PD was achieved via suppression of reactive astrocyte and inhibition of microglial activation.
鱼藤酮是制作帕金森病（PD）动物模型最广泛使用的神经毒素。神经退行性疾病PD表现出运动迟缓、静止性震颤、僵硬、步态障碍和姿势不稳等症状。我们研究了跑步机跑步是否能改善鱼藤酮诱导的PD大鼠的运动能力。还评估了跑步机跑步对PD的影响与小脑浦肯野细胞凋亡的关系。
从出生后4周开始，对运动组的大鼠每天进行一次30分钟的跑步机跑步，持续4周。我们使用转棒试验来测定运动协调性和平衡能力。在本实验中，进行了末端脱氧核苷酸转移酶介导的dUTP缺口末端标记（TUNEL）染色、钙结合蛋白、胶质纤维酸性蛋白（GFAP）、离子钙结合衔接分子1（Iba-1）的免疫组织化学以及Bax和Bcl-2的蛋白质印迹分析。
跑步机跑步通过防止小脑蚓部浦肯野细胞的丢失来增强运动平衡和协调性。跑步机跑步抑制了PD诱导的GFAP阳性反应性星形胶质细胞和Iba-1阳性小胶质细胞的表达，表明跑步机跑步抑制了反应性星形胶质细胞增生和小胶质细胞活化。跑步机跑步抑制了TUNEL阳性细胞数量和Bax表达，并增强了Bcl-2表达，表明跑步机跑步抑制了鱼藤酮诱导的PD大鼠小脑凋亡的进程。
跑步机跑步通过抑制小脑凋亡改善了鱼藤酮诱导的PD大鼠的运动能力。跑步机跑步对鱼藤酮诱导的PD的凋亡抑制作用是通过抑制反应性星形胶质细胞和抑制小胶质细胞活化来实现的。
