Jung Sun-Young, Kim Dae-Young
Department of Physical Therapy, Hosan University, Gyeongsan, Korea.
Department of Sports Healthcare, College of Humanities & Social Sciences, Inje University, Gimhae, Korea.
J Exerc Rehabil. 2017 Apr 30;13(2):136-142. doi: 10.12965/jer.1734964.482. eCollection 2017 Apr.
Cerebral palsy (CP) is a chronic disorder characterized by physical disability and disruption of brain function. We evaluated the effects of treadmill exercise on motor and memory functions in relation with phosphatidylinositol 3-kinase (PI3K)-Akt pathway using CP rat model. Rota-rod test, step-down avoidance task, 5-bromo-2'-deoxyuridine (BrdU) immunohistochemistry, and western blot for synapsin I, postsynaptic density-95 (PSD-95), PI3K, Akt, and glycogen synthase kinase-3β (GSK-3β) were performed. CP was induced by maternal lipopolysaccharide (LPS)-injection with sensorimotor restriction. Five weeks after birth, the rats in the exercise groups were made to run on the treadmill for 30 min per one day, 5 times a week, during 4 weeks. Motor and memory functions were impaired in the LPS-induced CP rats and tread-mill exercise increased motor and memory functions in the CP rats. Cell proliferation in the hippocampus was suppressed in the LPS-induced CP rats and treadmill exercise increased hippocampal cell proliferation in the CP rats. Expressions of synapsin I, PSD-95, phosphorylated (p)-PI3K, and p-Akt were decreased in the LPS-induced CP rats and treadmill exercise enhanced the expressions of synapsin I, PSD-95, p-PI3K, and p-Akt in the CP rats. GSK-3β expression was increased in the LPS-induced CP rats and treadmill exercise suppressed GSK-3β expression in the CP rats. The present results suggest that treadmill exercise might improve motor and memory functions through activation of PI3K-Akt pathway.
脑瘫（CP）是一种以身体残疾和脑功能紊乱为特征的慢性疾病。我们使用脑瘫大鼠模型评估了跑步机运动对与磷脂酰肌醇3-激酶（PI3K）-Akt信号通路相关的运动和记忆功能的影响。进行了转棒试验、避暗试验、5-溴-2'-脱氧尿苷（BrdU）免疫组化以及对突触素I、突触后致密蛋白95（PSD-95）、PI3K、Akt和糖原合酶激酶-3β（GSK-3β）的蛋白质印迹分析。通过母体注射脂多糖（LPS）并限制感觉运动诱导脑瘫。出生五周后，运动组的大鼠在四周内每周五天每天在跑步机上跑步30分钟。LPS诱导的脑瘫大鼠的运动和记忆功能受损，而跑步机运动增强了脑瘫大鼠的运动和记忆功能。LPS诱导的脑瘫大鼠海马中的细胞增殖受到抑制，而跑步机运动增加了脑瘫大鼠海马中的细胞增殖。LPS诱导的脑瘫大鼠中突触素I、PSD-95、磷酸化（p）-PI3K和p-Akt的表达降低，而跑步机运动增强了脑瘫大鼠中突触素I、PSD-95、p-PI3K和p-Akt的表达。LPS诱导的脑瘫大鼠中GSK-3β表达增加，而跑步机运动抑制了脑瘫大鼠中GSK-3β的表达。目前的结果表明，跑步机运动可能通过激活PI3K-Akt信号通路改善运动和记忆功能。
