Ahlfors K, Forsgren M, Griffiths P, Nielsen C M
Scand J Infect Dis. 1987;19(3):303-8. doi: 10.3109/00365548709018475.
Four serological tests (3 immunoassays using enzyme-labelled antigen and 1 radioimmunoassay) were compared as regards the detection of cytomegalovirus (CMV) immunoglobulin M in cord sera. 68 cord sera from infants congenitally infected by CMV were included in the study. The infections were primarily diagnosed by virus isolation close to birth. Four laboratories in 3 countries were involved, each laboratory using its own or a commercial test. Of the sera tested in the different laboratories 50-80% were found to be reactive. Both qualitatively and quantitatively there was a good correlation between the 3 enzyme-immunoassays. The RIA results differed to some extent from the enzyme tests as regards the quantification of IgM. The advantage of prospective IgM screening in undiluted cord sera followed by confirmatory virus isolation test in the neonatal period in IgM-positive cases is discussed.
对四种血清学检测方法（3种使用酶标抗原的免疫测定法和1种放射免疫测定法）在检测脐带血清中巨细胞病毒（CMV）免疫球蛋白M方面进行了比较。本研究纳入了68份来自先天性感染CMV婴儿的脐带血清。感染主要在出生时通过病毒分离诊断。有3个国家的4个实验室参与其中，每个实验室使用自己的或商业检测方法。在不同实验室检测的血清中，发现50%-80%呈反应性。3种酶免疫测定法在定性和定量方面都有良好的相关性。就IgM的定量而言，放射免疫测定法的结果与酶检测法在一定程度上有所不同。讨论了在未稀释的脐带血清中进行前瞻性IgM筛查，随后对IgM阳性病例在新生儿期进行确诊病毒分离检测的优势。
