Cdh1 的降解是由 APC/C-Cdh1 和 SCF-Cdc4 在出芽酵母中介导的。
Cdh1 degradation is mediated by APC/C-Cdh1 and SCF-Cdc4 in budding yeast.
机构信息
Department of Biological Science, Graduate School of Integrated Science and Technology, Shizuoka University, Shizuoka, 422-8529, Japan.
Department of Biological Science, Faculty of Science, Shizuoka University, Shizuoka, 422-8529, Japan.
出版信息
Biochem Biophys Res Commun. 2018 Dec 2;506(4):932-938. doi: 10.1016/j.bbrc.2018.10.179. Epub 2018 Nov 2.
Cdh1, a substrate-recognition subunit of anaphase-promoting complex/cyclosome (APC/C), is a tumor suppressor, and it is downregulated in various tumor cells in humans. APC/C-Cdh1 is activated from late M phase to G1 phase by antagonizing Cdk1-mediated inhibitory phosphorylation. However, how Cdh1 protein levels are properly regulated is ill-defined. Here we show that Cdh1 is degraded via APC/C-Cdh1 and Skp1-Cullin1-F-box (SCF)-Cdc4 in the budding yeast Saccharomyces cerevisiae. Cdh1 degradation was promoted by forced localization of Cdh1 into the nucleus, where APC/C and SCF are present. Cdk1 promoted APC/C-Cdh1-mediated Cdh1 degradation, whereas polo kinase Cdc5 elicited SCF-Cdc4-mediated degradation. Thus, Cdh1 degradation is controlled via multiple pathways.
Cdh1 是后期促进复合物/细胞周期蛋白（APC/C）的底物识别亚基，是一种肿瘤抑制因子，在人类的各种肿瘤细胞中均下调。APC/C-Cdh1 通过拮抗 Cdk1 介导的抑制性磷酸化，从晚期 M 期到 G1 期被激活。然而，Cdh1 蛋白水平是如何被正确调节的尚不清楚。在这里，我们表明 Cdh1 在 budding 酵母 Saccharomyces cerevisiae 中通过 APC/C-Cdh1 和 Skp1-Cullin1-F-box (SCF)-Cdc4 进行降解。Cdh1 的降解是通过强迫将 Cdh1 定位到核内来促进的，APC/C 和 SCF 都存在于核内。Cdk1 促进了 APC/C-Cdh1 介导的 Cdh1 降解，而 Polo 激酶 Cdc5 则引发了 SCF-Cdc4 介导的降解。因此，Cdh1 的降解是通过多种途径控制的。
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