State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, Hubei, China.
Key Laboratory of Development of Veterinary Diagnostic Products of Ministry of Agricultural, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, Hubei, China.
Sci Rep. 2018 Nov 5;8(1):16364. doi: 10.1038/s41598-018-34695-y.
Human carboxylesterases has been proven to be age and race-related and a sound basis of clinical medication. PLE involve in signal transduction and highly catalyze hydrolysis. Therefore, the expression level of PLE most probably exist age and breed difference and lead to significant differences of pharmacology and physiology. Four age groups of Tongcheng (TC) and Large White (LW) pigs were selected to explore PLE breed and age differences, and it was found that PLE mRNA was most abundant in liver in both breeds. In liver, PLE levels and hydrolytic activities increased with age, and PLE levels (except for 3 month) and the hydrolytic activities were higher in LW than in TC across all age groups. Abundance of PLE isoenzymes was obvious different between breeds and among age groups. The most abundant PLE isoenzyme in LW and TC pigs was PLE-A1 (all age groups) and PLE-B9 (three early age groups) or PLE-G3 (adult groups), respectively. 103 new PLE isoenzymes were found, and 55 high-frequency PLE isoenzymes were accordingly classified into seven categories (A-G). The results of this research provide a necessary basis not only for clinical medication of pigs but also for pig breeding purposes.
人类羧酸酯酶已被证明与年龄和种族有关，这为临床用药提供了可靠的依据。PLE 参与信号转导，并高度催化水解。因此，PLE 的表达水平很可能存在年龄和品种差异，导致药理学和生理学上的显著差异。本研究选择了四个年龄组的通城（TC）和大白猪（LW）来探讨 PLE 的品种和年龄差异，结果发现两种品种的 PLE mRNA 在肝脏中表达最丰富。在肝脏中，PLE 水平和水解活性随年龄增长而增加，在所有年龄组中，LW 的 PLE 水平（除 3 月龄外）和水解活性均高于 TC。PLE 同工酶的丰度在品种间和年龄组间存在明显差异。LW 和 TC 猪最丰富的 PLE 同工酶分别为 PLE-A1（所有年龄组）和 PLE-B9（三个早期年龄组）或 PLE-G3（成年组）。共发现了 103 种新的 PLE 同工酶，其中 55 种高频 PLE 同工酶被相应地分为 7 类（A-G）。本研究结果不仅为猪的临床用药提供了必要的依据，也为猪的选育提供了依据。
