Kadkhoda Zainab, Rafiei Sahar Chokami, Azizi Bahare, Khoshzaban Ahad
Associate Professor, Department of Peridontology, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran.
Postgraduate Student, Department of Periodontology, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran.
J Dent (Tehran). 2016 Sep;13(5):325-332.
Periodontal tissue regeneration for treatment of periodontal disease has not yet been mastered in tissue engineering. Stem cells, scaffold, and growth factors are the three main basic components of tissue engineering. Periodontal ligament (PDL) contains stem cells; however, the number, potency and features of these cells have not yet been understood. This study aimed to isolate and characterize the properties of PDL stem cells.
In this experimental study, samples were isolated from the PDL of extracted teeth of five patients and then stained immunohistochemically for detection of cell surface markers. Cells were then examined by immuno-flow cytometry for mesenchymal markers as well as for osteogenic and adipogenic differentiation.
The isolated cell population had fibroblast-like morphology and flow cytometry revealed that the mesenchymal surface markers were (means): CD90 (84.55), CD31 (39.97), CD166 (33.77), CD105 (31.19), CD45 (32/44), CD44 (462.11), CD34 (227.33), CD38 (86.94), CD13 (34.52) and CD73 (50.39). The PDL stem cells also differentiated into osteoblasts and adipocytes in osteogenic and adipogenic media, respectively.
PDL stem cells expressed mesenchymal stem cell (MSC) markers and differentiated into osteoblasts and adipocytes in osteogenic and adipogenic media, respectively.
在组织工程中，用于治疗牙周疾病的牙周组织再生技术尚未成熟。干细胞、支架和生长因子是组织工程的三个主要基本组成部分。牙周韧带（PDL）含有干细胞；然而，这些细胞的数量、潜能和特性尚未明确。本研究旨在分离并鉴定PDL干细胞的特性。
在本实验研究中，从5名患者拔除牙齿的PDL中分离样本，然后进行免疫组织化学染色以检测细胞表面标志物。接着通过免疫流式细胞术检测细胞的间充质标志物以及成骨和成脂分化情况。
分离出的细胞群体具有成纤维细胞样形态，流式细胞术显示间充质表面标志物（平均值）为：CD90（84.55）、CD31（39.97）、CD166（33.77）、CD105（31.19）、CD45（32/44）、CD44（462.11）、CD34（227.33）、CD38（86.94）、CD13（34.52）和CD73（50.39）。PDL干细胞在成骨和成脂培养基中分别分化为成骨细胞和脂肪细胞。
PDL干细胞表达间充质干细胞（MSC）标志物，并在成骨和成脂培养基中分别分化为成骨细胞和脂肪细胞。
