Nabil Nehal M, Tawakol Maram M, Hassan Heba M
Reference Laboratory for Veterinary Quality control on Poultry production, Animal health research institute, Giza, Egypt.
Infect Ecol Epidemiol. 2018 Oct 30;8(1):1539056. doi: 10.1080/20008686.2018.1539056. eCollection 2018.
Salmonellosis is one of the main bacterial infections affecting commercial poultry, causing losses to poultry production, and posing a public health concern. Samples from internal organs (liver, cecum and spleen) of one hundred diseased broiler chickens were collected and subjected to isolation, identification and serotyping. and were selected from the isolated to prepare bacteriophages from sewage water taken at broiler farms. An experimental infection of one day old specific pathogen free (SPF) chicks followed by treatment with the prepared bacteriophages isolated from both was performed. Caecal samples from infected chicks were subjected at intervals to bacteriophage isolation and quantitation. The effectiveness of bacteriophage treatments on colonization in cecum of infected chicks increased after five successive doses. At 3 day post infection (dpi), cecal contents showed a marginal decrease in Salmonella loads with more reduction at 5 dpi. From 7 dpi to the end of the experiment at 15 dpi, all the chicks were cleared for both . The findings of this study demonstrate that bacteriophage treatment is efficacious in reducing and colonization in broiler chickens within a short period and could be used as an alternative to antibiotics.
沙门氏菌病是影响商业家禽的主要细菌感染之一，会给家禽生产造成损失，并引发公共卫生问题。收集了100只患病肉鸡的内脏器官（肝脏、盲肠和脾脏）样本，进行分离、鉴定和血清分型。从分离出的菌株中挑选菌株，从肉鸡场采集的污水中制备噬菌体。对1日龄无特定病原体（SPF）雏鸡进行实验性感染，然后用从两种菌株中分离出的制备好的噬菌体进行治疗。对感染雏鸡的盲肠样本定期进行噬菌体分离和定量分析。连续五次给药后，噬菌体治疗对感染雏鸡盲肠中两种菌株定植的有效性增加。在感染后3天（dpi），盲肠内容物中的沙门氏菌载量略有下降，在5 dpi时下降更多。从7 dpi到实验结束时的15 dpi，所有雏鸡的两种菌株均被清除。本研究结果表明，噬菌体治疗在短期内有效减少肉鸡中的两种菌株及其定植，可作为抗生素的替代品。
