Department of Genetics and Genome Biology, University of Leicester, Leicester, UK.
Ab Agri, Innovation Way, Peterborough Business Park, Peterborough, UK.
Emerg Microbes Infect. 2023 Dec;12(1):2217947. doi: 10.1080/22221751.2023.2217947.
Nontyphoidal spp. are a leading cause of human gastrointestinal infections and are commonly transmitted via the consumption of contaminated meat. To limit the spread of and other food-borne pathogens in the food chain, bacteriophage (phage) therapy could be used during rearing or pre-harvest stages of animal production. This study was conducted to determine if a phage cocktail delivered in feed is capable of reducing colonization in experimentally challenged chickens and to determine the optimal phage dose. A total of 672 broilers were divided into six treatment groups T1 (no phage diet and unchallenged); T2 (phage diet 10 PFU/day); T3 (challenged group); T4 (phage diet 10 PFU/day and challenged); T5 (phage diet 10 PFU/day and challenged); and T6 (phage diet 10 PFU/day and challenged). The liquid phage cocktail was added to mash diet with access available throughout the study. By day 42 (the concluding day of the study), no was detected in faecal samples collected from group T4. was isolated from a small number of pens in groups T5 (3/16) and T6 (2/16) at ∼4 × 10 CFU/g. In comparison, was isolated from 7/16 pens in T3 at ∼3 × 10 CFU/g. Phage treatment at all three doses had a positive impact on growth performance in challenged birds with increased weight gains in comparison to challenged birds with no phage diet. We showed delivering phages via feed was effective at reducing colonization in chickens and our study highlights phages offer a promising tool to target bacterial infections in poultry.
非伤寒沙门氏菌是人类胃肠道感染的主要原因，通常通过食用受污染的肉类传播。为了限制在食物链中传播和其他食源性病原体，可以在动物生产的饲养或收获前阶段使用噬菌体（噬菌体）疗法。本研究旨在确定在饲料中递送噬菌体鸡尾酒是否能够减少实验性攻毒鸡中的定植，并确定最佳噬菌体剂量。将 672 只肉鸡分为 6 个处理组 T1（无噬菌体饮食和未攻毒）；T2（噬菌体饮食 10 PFU/天）；T3（攻毒组）；T4（噬菌体饮食 10 PFU/天和攻毒）；T5（噬菌体饮食 10 PFU/天和攻毒）和 T6（噬菌体饮食 10 PFU/天和攻毒）。液体噬菌体鸡尾酒被添加到糊料饮食中，在整个研究过程中均可获得。到第 42 天（研究结束日），未从 T4 组收集的粪便样本中检测到。在 T5（3/16）和 T6（2/16）组的少数几个鸡舍中分离到少量。相比之下，在 T3 组的 7/16 个鸡舍中分离到。噬菌体治疗在所有三个剂量下都对攻毒鸡的生长性能产生了积极影响，与未接受噬菌体饮食的攻毒鸡相比，攻毒鸡的体重增加。我们表明，通过饲料递送噬菌体可有效减少鸡中的定植，我们的研究强调噬菌体为靶向家禽中的细菌感染提供了有前途的工具。
