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通过冷冻电子显微镜确定的细胞色素c亚硝酸还原酶的三维结构

Three-Dimensional Structure of Cytochrome c Nitrite Reductase As Determined by Cryo-Electron Microscopy.

作者信息

Baymukhametov T N, Chesnokov Y M, Pichkur E B, Boyko K M, Tikhonova T V, Myasnikov A G, Vasiliev A L, Lipkin A V, Popov V O, Kovalchuk M V

机构信息

National Research Center «Kurchatov Institute», Akademika Kurchatova Sqr., 1, Moscow, 123182 , Russia.

Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, Leninsky Ave., 33, bldg. 2, Moscow, 119071, Russia.

出版信息

Acta Naturae. 2018 Jul-Sep;10(3):48-56.

PMID:30397526
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6209398/
Abstract

The structure of cytochrome c nitrite reductase from the bacterium Thioalkalivibrio nitratireducens was determined by cryo-electron microscopy (cryo-EM) at a 2.56 Å resolution. Possible structural heterogeneity of the enzyme was assessed. The backbone and side-chain orientations in the cryo-EM-based model are, in general, similar to those in the high-resolution X-ray diffraction structure of this enzyme.

摘要

通过冷冻电子显微镜（cryo-EM）在2.56埃分辨率下确定了来自嗜碱硫杆菌硝酸还原菌的细胞色素c亚硝酸盐还原酶的结构。评估了该酶可能存在的结构异质性。基于冷冻电子显微镜的模型中的主链和侧链取向通常与该酶的高分辨率X射线衍射结构中的取向相似。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2493/6209398/25b956adaeae/AN20758251-10-3-048-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2493/6209398/5d8bba2c9869/AN20758251-10-3-048-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2493/6209398/92f1affca667/AN20758251-10-3-048-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2493/6209398/92c86147fd68/AN20758251-10-3-048-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2493/6209398/c3bda6890729/AN20758251-10-3-048-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2493/6209398/a28b44ed2cca/AN20758251-10-3-048-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2493/6209398/30146cad8d13/AN20758251-10-3-048-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2493/6209398/a933ddbbde65/AN20758251-10-3-048-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2493/6209398/25b956adaeae/AN20758251-10-3-048-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2493/6209398/5d8bba2c9869/AN20758251-10-3-048-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2493/6209398/92f1affca667/AN20758251-10-3-048-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2493/6209398/92c86147fd68/AN20758251-10-3-048-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2493/6209398/c3bda6890729/AN20758251-10-3-048-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2493/6209398/a28b44ed2cca/AN20758251-10-3-048-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2493/6209398/30146cad8d13/AN20758251-10-3-048-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2493/6209398/a933ddbbde65/AN20758251-10-3-048-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2493/6209398/25b956adaeae/AN20758251-10-3-048-g008.jpg

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