School of Chemistry, University of New South Wales, Sydney, NSW, 2052, Australia.
Department of Inorganic and Analytical Chemistry, University of Geneva, Quai E.-Ansermet 30, 1211, Geneva 4, Switzerland.
Angew Chem Int Ed Engl. 2018 Dec 17;57(51):16801-16805. doi: 10.1002/anie.201811268. Epub 2018 Nov 27.
We report here on a light addressable potassium (K ) sensor where light illumination of a semiconducting silicon electrode substrate results in a localized activation of the faradaic electrochemistry at the illuminated spot. This allows one, by electrochemical control, to oxidize surface bound ferrocene moieties that in turn trigger K transfer from the overlaid K -selective film to the solution phase. The resulting voltammetric response is shown to be K -selective, where peak position is a direct function of K activity at the surface of electrode. This concept was used to measure extracellular K concentration changes by stimulating living breast cancer cells. The associated decrease of intracellular K level was confirmed with a fluorescent K indicator. In contrast to light addressable potentiometry, the approach introduced here relies on dynamic electrochemistry and may be performed in tandem with other electrochemical analysis when studying biological events on the electrode.
我们在此报告一种光寻址钾（K）传感器，其中半导体硅电极衬底的光照射导致在照射点处的法拉第电化学的局部激活。通过电化学控制，这使得能够氧化表面结合的二茂铁部分，其反过来触发从覆盖的 K 选择膜到溶液相的 K 转移。所得到的伏安响应被证明是 K 选择性的，其中峰位置是电极表面处 K 活性的直接函数。该概念用于通过刺激活乳腺癌细胞来测量细胞外 K 浓度变化。用荧光 K 指示剂证实了细胞内 K 水平的相应降低。与光寻址电位法不同，此处介绍的方法依赖于动态电化学，并且当在电极上研究生物事件时，可以与其他电化学分析同时进行。
