Department of Food Science and Engineering , Harbin Institute of Technology , No 92 West Dazhi Street , Harbin 150001 , People's Republic of China.
Center of Drugs Safety and Evaluation , Heilongjiang University of Chinese Medicine , Harbin , People's Republic of China.
J Agric Food Chem. 2018 Dec 5;66(48):12696-12705. doi: 10.1021/acs.jafc.8b04874. Epub 2018 Nov 16.
The viable bacterial strains in conventional yogurt are intolerant to bile acid, which consequently cannot survive the conditions and their beneficial bioactivities are thus lost. We have previously shown that Lactobacillus casei Q14 ( Lac-Q14), a probiotic, has the potential to alleviate diabetes in rats. Herein, we used Lac-Q14 as the starter culture to ferment yogurt and explore the mechanisms of the bioactivity in diabetic rats. The results showed that Lac-Q14 yogurt improved blood glucose and insulin level, lowered gene expression of critical enzymes involved in liver gluconeogenesis. Pyrosequencing showed an obvious change in the composition of intestinal microbiota in Lac-Q14 yogurt treated rats. The abundance of 21 genera differed significantly between the Lac-Q14 yogurt group and diabetes group. Quite a few short-chain fatty acid (SCFA)-producing bacteria were selectively enriched, along with increased concentrations of SCFA and downstream Glucagon-like peptide-1 (GLP-1) and Peptide YY (PYY) secretion.
常规酸奶中的活菌菌株不能耐受胆汁酸，因此无法在这些条件下存活，其有益的生物活性也随之丧失。我们之前已经表明，益生菌干酪乳杆菌 Q14（Lac-Q14）具有缓解大鼠糖尿病的潜力。在此，我们使用 Lac-Q14 作为发酵酸奶的起始培养物，并探索其在糖尿病大鼠中的生物活性机制。结果表明，Lac-Q14 酸奶改善了血糖和胰岛素水平，降低了参与肝糖异生的关键酶的基因表达。焦磷酸测序显示，Lac-Q14 酸奶处理的大鼠肠道微生物群组成发生了明显变化。Lac-Q14 酸奶组和糖尿病组之间有 21 个属的丰度有显著差异。大量的短链脂肪酸（SCFA）产生菌被选择性富集，同时 SCFA 及其下游胰高血糖素样肽-1（GLP-1）和肽 YY（PYY）的分泌浓度也增加。
