College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, China.
Food Funct. 2019 May 22;10(5):2935-2946. doi: 10.1039/c9fo00475k.
In the present study, we aimed to investigate the therapeutic mechanisms of carrot juice fermented with Lactobacillus rhamnosus GG (LGG) on type 2 diabetic mellitus (T2DM) based on the regulation of gut microbiota. Carrot juice fermented with LGG was enriched with free phenols, organic acids and short-chain fatty acids (SCFAs). Supplementation of carrot juice fermented with LGG (DFCL) could favorably regulate blood glucose, insulin, antioxidant capacity and morphology of the pancreas and kidney in the diabetic rats, accompanied by an increase of SCFAs in the cecum. Furthermore, high-throughput sequencing (HTS) analysis revealed that DFCL supplementation altered the composition of gut microbiota, showing increased relative abundances of functionally relevant enterotypes, such as Christensenellaceae_R-7_group, Oscillibacter, Ruminococcaceae_UCG-013, Lachnospiraceae_NK4A136_group and Akkermansia. In addition, Spearman's correlation analysis revealed that Desulfovibrio, Ruminococcaceae and Alloprevotella were closely correlated with biochemical biomarkers. Meanwhile, DFCL treatment regulated the expressions of genes involved in glucose metabolism at the mRNA and protein levels.
在本研究中，我们旨在基于肠道微生物群的调节，研究鼠李糖乳杆菌 GG（LGG）发酵胡萝卜汁对 2 型糖尿病（T2DM）的治疗机制。LGG 发酵的胡萝卜汁富含游离酚类、有机酸和短链脂肪酸（SCFAs）。补充 LGG 发酵的胡萝卜汁（DFCL）可使糖尿病大鼠的血糖、胰岛素、抗氧化能力和胰腺及肾脏形态得到有利调节，同时盲肠中 SCFAs 增加。此外，高通量测序（HTS）分析显示，DFCL 补充改变了肠道微生物群的组成，表现出与功能相关的肠型（如 Christensenellaceae_R-7_group、Oscillibacter、Ruminococcaceae_UCG-013、Lachnospiraceae_NK4A136_group 和 Akkermansia）相对丰度增加。此外，Spearman 相关性分析显示，脱硫弧菌、瘤胃球菌科和 Alloprevotella 与生化标志物密切相关。同时，DFCL 处理调节了参与葡萄糖代谢的基因在 mRNA 和蛋白水平的表达。
